The VHL (Von Hippel-Lindau) Polyclonal Antibody (PACO37082) is a valuable tool for researchers studying the VHL protein, a key player in regulating the stability of hypoxia-inducible factor (HIF) and oxygen sensing in cells. This antibody, produced in rabbits, exhibits high reactivity with human samples and has been validated for use in Western blot applications.The VHL protein is known for its role in the ubiquitin-mediated degradation of HIF, an essential transcription factor that orchestrates cellular responses to low oxygen levels. Dysregulation of the VHL-HIF pathway has been implicated in various diseases, including cancer, neurodegenerative disorders, and cardiovascular conditions.
By targeting the VHL protein, researchers can gain insights into the mechanisms underlying these diseases and identify potential therapeutic strategies.With its specificity and sensitivity, the VHL Polyclonal Antibody is a versatile tool for investigating VHL protein expression and function in different cell types and experimental settings. Its use in immunology, oncology, and cell biology research makes it an indispensable asset for scientists seeking to unravel the complexities of oxygen sensing and HIF regulation.
Antibody Name:
VHLL Antibody (PACO37082)
Antibody SKU:
PACO37082
Size:
50ug
Host Species:
Rabbit
Tested Applications:
ELISA, IHC
Recommended Dilutions:
ELISA:1:2000-1:10000, IHC:1:20-1:200
Species Reactivity:
Human
Immunogen:
Recombinant Human von Hippel-Lindau-like protein (1-139AA)
Immunohistochemistry of paraffin-embedded human placenta tissue using PACO37082 at dilution of 1:100.
Immunohistochemistry of paraffin-embedded human endometrial cancer using PACO37082 at dilution of 1:100.
Background:
Functions as a dominant-negative VHL to serve as a protector of HIFalpha.
Synonyms:
von Hippel-Lindau-like protein (VHL-like protein) (VLP), VHLL, VLP
UniProt Protein Function:
Functions as a dominant-negative VHL to serve as a protector of HIFalpha.
UniProt Protein Details:
NCBI Summary:
Von Hippel-Lindau (VHL) tumor suppressor protein is a component of an E3 ubiquitin ligase complex that selectively ubiquitinates the alpha subunit of the hypoxia-inducible factor (HIF) transcription factor for proteasome-mediated degradation. Inactivation of VHL causes VHL disease and sporadic kidney cancer. This gene encodes a VHL homolog that lacks one of two key domains necessary for VHL function. This gene may contribute to the regulation of oxygen homeostasis and neovascularization during placenta development. This gene is intronless, and can also be interpreted as a retrotransposed pseudogene of the VHL locus located on chromosome 3. However, the protein is represented in this RefSeq due to evidence in PMID:14757845 that strongly suggests it is translated. The same publication also indicates that this protein binds HIF alpha but fails to recruit the E3 ubiquitin ligase complex, and it therefore functions as a dominant-negative VHL protein and a protector of HIF alpha. [provided by RefSeq, Jan 2010]
