The VHL Polyclonal Antibody (CAB16287) is a crucial tool for research involving the Von Hippel-Lindau (VHL) protein, a tumor suppressor that plays a key role in regulating cell growth and division. This antibody, generated in rabbits, is highly specific for human samples and has been validated for use in Western blot applications. By binding to the VHL protein, this antibody enables researchers to detect and analyze VHL expression in various cell types, making it an essential resource for studies in cancer biology and molecular oncology.The VHL protein is known for targeting hypoxia-inducible factors (HIFs) for degradation, thereby controlling the expression of genes involved in angiogenesis and cell proliferation.
Dysregulation of VHL function is a hallmark of clear cell renal cell carcinoma and other VHL-associated diseases. Research on the VHL protein is vital for understanding the mechanisms underlying tumorigenesis and for developing targeted therapies for VHL-related cancers.By using the VHL Polyclonal Antibody (CAB16287), researchers can further elucidate the molecular pathways governed by the VHL protein, leading to valuable insights into cancer progression and potential therapeutic interventions. This antibody is a valuable tool for investigating the role of VHL in tumor development and progression, paving the way for new strategies in cancer treatment.
Product Name:
VHL Rabbit Polyclonal Antibody
SKU:
CAB16287
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Mouse,Rat
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 1-100 of human VHL (NP_937799.1).
This gene encodes a component of a ubiquitination complex. The encoded protein is involved in the ubiquitination and degradation of hypoxia-inducible-factor (HIF), which is a transcription factor that plays a central role in the regulation of gene expression by oxygen. In addition to oxygen-related gene expression, this protein plays a role in many other cellular processes including cilia formation, cytokine signaling, regulation of senescence, and formation of the extracellular matrix. Variants of this gene are associated with von Hippel-Lindau syndrome, pheochromocytoma, erythrocytosis, renal cell carcinoma, and cerebellar hemangioblastoma.
Purification Method:
Affinity purification
Gene ID:
7428
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using VHL Rabbit pAb (CAB16287) at 1:3000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s.
