The VHL Polyclonal Antibody (CAB0377) is a valuable tool for researchers studying the von Hippel-Lindau (VHL) tumor suppressor protein. This antibody, produced in rabbits, is highly specific to human VHL protein and has been validated for use in Western blot applications. By binding to the VHL protein, this antibody enables the detection and analysis of VHL in various cell types, making it an ideal choice for studies in cancer research and molecular biology.The VHL protein is known for its role in regulating the stability of hypoxia-inducible factors (HIFs) and plays a crucial role in oxygen sensing and angiogenesis.
Mutations in the VHL gene are associated with the development of VHL disease, a hereditary cancer syndrome characterized by the formation of tumors in various organs. Understanding the function of the VHL protein is essential for unraveling the mechanisms of tumor development and progression, making the VHL Polyclonal Antibody a valuable asset for researchers in the field.
Product Name:
VHL Rabbit Polyclonal Antibody
SKU:
CAB0377
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 87-186 of human VHL (NP_000542.1).
This gene encodes a component of a ubiquitination complex. The encoded protein is involved in the ubiquitination and degradation of hypoxia-inducible-factor (HIF), which is a transcription factor that plays a central role in the regulation of gene expression by oxygen. In addition to oxygen-related gene expression, this protein plays a role in many other cellular processes including cilia formation, cytokine signaling, regulation of senescence, and formation of the extracellular matrix. Variants of this gene are associated with von Hippel-Lindau syndrome, pheochromocytoma, erythrocytosis, renal cell carcinoma, and cerebellar hemangioblastoma.
Purification Method:
Affinity purification
Gene ID:
7428
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3.
Western blot analysis of various lysates using VHL Rabbit pAb (CAB0377) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 30s.
