The VD (Vitamin D) ELISA Kit is a highly reliable and accurate tool for the quantification of vitamin D levels in human serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, providing consistent and precise results for various research applications related to vitamin D analysis.Vitamin D is a critical nutrient that plays a vital role in bone health, immune function, and overall well-being. Deficiencies in vitamin D have been associated with a range of health issues, including osteoporosis, cardiovascular diseases, and autoimmune disorders.
Therefore, monitoring vitamin D levels is essential for understanding and addressing these health concerns.Whether studying the effects of vitamin D supplementation, evaluating its impact on disease progression, or exploring its role in various physiological processes, the VD ELISA Kit is an invaluable tool for researchers seeking to unravel the complexities of vitamin D metabolism and its implications for human health.
Product Name:
VD (Vitamin D) ELISA Kit (UNES00009)
SKU:
UNES00009
Target:
VD (Vitamin D) (UNES00009)
Size:
96T
Assay type:
Competitive-ELISA
Assay time:
2.0h
Sensitivity:
3.75 ng/mL
Detection range:
6.25-400 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with VD. During the reaction, VD in the sample or standard competes with a fixed amount of VD on the solid phase supporter for sites on the Biotinylated Detection Ab specific to VD. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of VD in tested samples can be calculated by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
93-105
94-109
84-95
Average (%)
98
100
90
1:4
Range (%)
92-108
91-104
86-100
Average (%)
100
96
93
1:8
Range (%)
89-101
95-106
81-90
Average (%)
95
100
85
1:16
Range (%)
90-101
94-108
84-100
Average (%)
95
99
91
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
96-112
102
EDTA plasma (n=5)
88-99
93
Cell culture media (n=5)
85-97
91
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
21.62
53.66
157.91
21.41
57.3
152.81
Standard deviation
1.28
2.7
5.98
1.47
2.35
8.34
C V (%)
5.92
5.03
3.79
6.87
4.1
5.46
Sample type &Sample volume:
serum, plasma and other biological fluids; 50μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of VD concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes VD in samples. No significant cross-reactivity or interference between VD and analogues was observed.
