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VCP (Phospho-Ser352) Colorimetric Cell-Based ELISA Kit (CBCAB01693)

SKU:
CBCAB01693
Product Type:
ELISA Kit
ELISA Type:
Cell Based Phospho Specific
Research Area:
Autophagy
Reactivity:
Human
Mouse
Rat
Detection Method:
Colorimetric
€960

Description

system_update_altDatasheetMSDS

VCP (Phospho-Ser352)Colorimetric Cell-Based ELISA Kit

The VCP Phospho-Ser352 Colorimetric Cell-Based ELISA Kit is a cutting-edge tool designed for the accurate detection of phosphorylated valosin-containing protein (VCP) at serine 352 in cell lysates. This kit offers high sensitivity and specificity, ensuring reliable and reproducible results for researchers studying the role of VCP in cellular processes.VCP is a key protein involved in various cellular functions, including protein degradation and quality control. Phosphorylation of VCP at serine 352 has been linked to its regulation and function, making it a crucial target for understanding cellular signaling pathways and disease mechanisms.

By using the VCP Phospho-Ser352 Colorimetric Cell-Based ELISA Kit, researchers can confidently analyze VCP phosphorylation levels in cell-based assays, providing valuable insights into the mechanisms underlying protein quality control, neurodegenerative diseases, and other pathologies. This kit is essential for advancing research in the field of cellular biology and drug development.

Product Name:VCP (Phospho-Ser352) Colorimetric Cell-Based ELISA
Product Code:CBCAB01693
ELISA Type:Cell-Based
Target:VCP (Phospho-Ser352)
Reactivity:Human, Mouse, Rat
Dynamic Range:> 5000 Cells
Detection Method:Colorimetric 450 nm
Format:2 x 96-Well Microplates

The VCP (Phospho-Ser352) Colorimetric Cell-Based ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can detect VCP protein phosphorylation and expression profile in cells. The kit can be used for measuring the relative amounts of phosphorylated VCP in cultured cells as well as screening for the effects that various treatments, inhibitors (ie. siRNA or chemicals), or activators have on VCP phosphorylation.

Qualitative determination of VCP (Phospho-Ser352) concentration is achieved by an indirect ELISA format. In essence, VCP (Phospho-Ser352) is captured by VCP (Phospho-Ser352)-specific primary (1ø) antibodies while the HRP-conjugated secondary (2ø) antibodies bind the Fc region of the 1ø antibody. Through this binding, the HRP enzyme conjugated to the 2ø antibody can catalyze a colorimetric reaction upon substrate addition. Due to the qualitative nature of the Cell-Based ELISA, multiple normalization methods are needed:

1. A monoclonal antibody specific for human GAPDH is included to serve as an internal positive control in normalizing the target absorbance values.
2. Following the colorimetric measurement of HRP activity via substrate addition, the Crystal Violet whole-cell staining method may be used to determine cell density. After staining, the results can be analysed by normalizing the absorbance values to cell amounts, by which the plating difference can be adjusted.
Database Information:Gene ID: 7415, UniProt ID: P55072, OMIM: 167320/601023, Unigene: Hs.529782/Hs.591084
Gene Symbol:VCP
Sub Type:Phospho
UniProt Protein Function:VCP: valosin-containing protein (VCP) is a member of a family that includes putative ATP-binding proteins involved in vesicle transport and fusion, 26S proteasome function, and assembly of peroxisomes. VCP, as a structural protein, is associated with clathrin, and heat-shock protein Hsc70, to form a complex. Necessary for the fragmentation of Golgi stacks during mitosis and for their reassembly after mitosis. Involved in the formation of the nuclear envelope and of the transitional endoplasmic reticulum (tER). Regulates NFKappaB pathway, which is important for metastasis of osteosarcoma. Tyrosine phosphorylation regulates its cell cycle-dependent nuclear localization.
UniProt Protein Details:

Protein type:Endoplasmic reticulum; EC 3.6.4.6; DNA repair, damage; Chaperone; Hydrolase

Chromosomal Location of Human Ortholog: 9p13.3

Cellular Component: nucleoplasm; proteasome complex; endoplasmic reticulum membrane; intracellular membrane-bound organelle; perinuclear region of cytoplasm; endoplasmic reticulum; cytoplasm; lipid particle; cytosol; nucleus

Molecular Function:protein domain specific binding; identical protein binding; protein binding; ATPase activity; protein complex binding; ADP binding; polyubiquitin binding; protein phosphatase binding; lipid binding; ATP binding; receptor binding

Biological Process: caspase activation; proteasomal ubiquitin-dependent protein catabolic process; ER to Golgi vesicle-mediated transport; ER-associated protein catabolic process; unfolded protein response; protein ubiquitination; DNA repair; regulation of apoptosis; retrograde protein transport, ER to cytosol; bypass DNA synthesis; establishment of protein localization; positive regulation of protein complex assembly; positive regulation of proteasomal ubiquitin-dependent protein catabolic process; double-strand break repair; positive regulation of protein catabolic process; protein amino acid N-linked glycosylation via asparagine; response to DNA damage stimulus; protein homooligomerization

Disease: Amyotrophic Lateral Sclerosis 14, With Or Without Frontotemporal Dementia; Inclusion Body Myopathy With Early-onset Paget Disease With Or Without Frontotemporal Dementia 1

NCBI Summary:The protein encoded by this gene is a member of a family that includes putative ATP-binding proteins involved in vesicle transport and fusion, 26S proteasome function, and assembly of peroxisomes. This protein, as a structural protein, is associated with clathrin, and heat-shock protein Hsc70, to form a complex. It has been implicated in a number of cellular events that are regulated during mitosis, including homotypic membrane fusion, spindle pole body function, and ubiquitin-dependent protein degradation. [provided by RefSeq, Jul 2008]
UniProt Code:P55072
NCBI GenInfo Identifier:6094447
NCBI Gene ID:7415
NCBI Accession:P55072.4
UniProt Secondary Accession:P55072,Q0V924, Q2TAI5, Q969G7, Q9UCD5, B2R5T8,
UniProt Related Accession:P55072
Molecular Weight:89,322 Da
NCBI Full Name:Transitional endoplasmic reticulum ATPase
NCBI Synonym Full Names:valosin containing protein
NCBI Official Symbol:VCP  
NCBI Official Synonym Symbols:p97; TERA; ALS14; IBMPFD  
NCBI Protein Information:transitional endoplasmic reticulum ATPase; TER ATPase; yeast Cdc48p homolog; valosin-containing protein; 15S Mg(2+)-ATPase p97 subunit
UniProt Protein Name:Transitional endoplasmic reticulum ATPase
UniProt Synonym Protein Names:15S Mg(2+)-ATPase p97 subunit; Valosin-containing protein
Protein Family:Transitional endoplasmic reticulum ATPase
UniProt Gene Name:VCP  
UniProt Entry Name:TERA_HUMAN
ComponentQuantity
96-Well Cell Culture Clear-Bottom Microplate2 plates
10X TBS24 mL
Quenching Buffer24 mL
Blocking Buffer50 mL
15X Wash Buffer50 mL
Primary Antibody Diluent12 mL
100x Anti-Phospho Target Antibody 60 µL
100x Anti-Target Antibody60 µL
Anti-GAPDH Antibody60 µL
HRP-Conjugated Anti-Rabbit IgG Antibody12 mL
HRP-Conjugated Anti-Mouse IgG Antibody12 mL
SDS Solution12 mL
Stop Solution24 mL
Ready-to-Use Substrate12 mL
Crystal Violet Solution12 mL
Adhesive Plate Seals2 seals

The following materials and/or equipment are NOT provided in this kit but are necessary to successfully conduct the experiment:

  • Microplate reader able to measure absorbance at 450 nm and/or 595 nm for Crystal Violet Cell Staining (Optional)
  • Micropipettes with capability of measuring volumes ranging from 1 µL to 1 ml
  • 37% formaldehyde (Sigma Cat# F-8775) or formaldehyde from other sources
  • Squirt bottle, manifold dispenser, multichannel pipette reservoir or automated microplate washer
  • Graph paper or computer software capable of generating or displaying logarithmic functions
  • Absorbent papers or vacuum aspirator
  • Test tubes or microfuge tubes capable of storing ≥1 ml
  • Poly-L-Lysine (Sigma Cat# P4832 for suspension cells)
  • Orbital shaker (optional)
  • Deionized or sterile water

*Note: Protocols are specific to each batch/lot. For the correct instructions please follow the protocol included in your kit.

StepProcedure
1.Seed 200 µL of 20,000 adherent cells in culture medium in each well of a 96-well plate. The plates included in the kit are sterile and treated for cell culture. For suspension cells and loosely attached cells, coat the plates with 100 µL of 10 µg/ml Poly-L-Lysine (not included) to each well of a 96-well plate for 30 minutes at 37 °C prior to adding cells.
2.Incubate the cells for overnight at 37 °C, 5% CO2.
3.Treat the cells as desired.
4.Remove the cell culture medium and rinse with 200 µL of 1x TBS, twice.
5.Fix the cells by incubating with 100 µL of Fixing Solution for 20 minutes at room temperature. The 4% formaldehyde is used for adherent cells and 8% formaldehyde is used for suspension cells and loosely attached cells.
6.Remove the Fixing Solution and wash the plate 3 times with 200 µL 1x Wash Buffer for five minutes each time with gentle shaking on the orbital shaker. The plate can be stored at 4 °C for a week.
7.Add 100 µL of Quenching Buffer and incubate for 20 minutes at room temperature.
8.Wash the plate 3 times with 1x Wash Buffer for 5 minutes each time.
9.Add 200 µL of Blocking Buffer and incubate for 1 hour at room temperature.
10. Wash 3 times with 200 µL of 1x Wash Buffer for 5 minutes each time.
11.Add 50 µL of 1x primary antibodies Anti-VCP (Phospho-Ser352) Antibody, Anti-VCP Antibody and/or Anti-GAPDH Antibody) to the corresponding wells, cover with Parafilm and incubate for 16 hours (overnight) at 4 °C. If the target expression is known to be high, incubate for 2 hours at room temperature.
12.Wash 3 times with 200 µL of 1x Wash Buffer for 5 minutes each time.
13.Add 50 µL of 1x secondary antibodies (HRP-Conjugated AntiRabbit IgG Antibody or HRP-Conjugated Anti-Mouse IgG Antibody) to corresponding wells and incubate for 1.5 hours at room temperature.
14.Wash 3 times with 200 µL of 1x Wash Buffer for 5 minutes each time.
15.Add 50 µL of Ready-to-Use Substrate to each well and incubate for 30 minutes at room temperature in the dark.
16.Add 50 µL of Stop Solution to each well and read OD at 450 nm immediately using the microplate reader.

(Additional Crystal Violet staining may be performed if desired – details of this may be found in the kit technical manual.)

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