The [KO Validated] VAT1 Antibody (CAB17077) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, developed using rabbits, is highly specific to human samples and is approved for use in Western blot applications. By binding to Vat1, this antibody enables researchers to detect and analyze the protein in various cell types, making it ideal for studies in cell biology and neuroscience.Vat1 is a key player in the transport of vesicles within cells, playing a crucial role in maintaining cellular function and communication.
This antibody is validated for use in WB, IF/ICC, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
[KO Validated] VAT1 Antibody
SKU:
CAB17077
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Synaptic vesicles are responsible for regulating the storage and release of neurotransmitters in the nerve terminal. The protein encoded by this gene is an abundant integral membrane protein of cholinergic synaptic vesicles and is thought to be involved in vesicular transport. It belongs to the quinone oxidoreductase subfamily of zinc-containing alcohol dehydrogenase proteins.
Purification Method
Affinity purification
Gene ID
10493
RRID
AB_2772842
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using [KO Validated] VAT1 Rabbit pAb (CAB17077) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 5min.
Western blot analysis of lysates from wild type (WT) and VAT1 knockout (KO) 293T cells, using [KO Validated] VAT1 Rabbit pAb (CAB17077) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 1s.
