The VASP Polyclonal Antibody (CAB14217) is a valuable tool for researchers studying Vasodilator-stimulated phosphoprotein (VASP), a key regulator of actin dynamics and cell migration. This antibody is generated in rabbits and is highly specific to human VASP protein, making it a reliable choice for Western blot applications. By binding to VASP, this antibody allows for the detection and analysis of VASP expression in various cell types, making it ideal for studies in cell biology, cancer metastasis, and cardiovascular research.VASP is a crucial protein involved in cellular processes such as cell adhesion, migration, and invasion, making it a target of interest in cancer progression and metastasis.
Additionally, VASP plays a role in regulating platelet aggregation and vasodilation, highlighting its importance in cardiovascular health. By investigating the function of VASP using this antibody, researchers can gain insights into its role in disease states and potentially develop targeted therapies for conditions such as cancer and cardiovascular disorders.
Product Name:
VASP Rabbit Polyclonal Antibody
SKU:
CAB14217
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 100-200 of human VASP (NP_003361.1).
Vasodilator-stimulated phosphoprotein (VASP) is a member of the Ena-VASP protein family. Ena-VASP family members contain an EHV1 N-terminal domain that binds proteins containing E/DFPPPPXD/E motifs and targets Ena-VASP proteins to focal adhesions. In the mid-region of the protein, family members have a proline-rich domain that binds SH3 and WW domain-containing proteins. Their C-terminal EVH2 domain mediates tetramerization and binds both G and F actin. VASP is associated with filamentous actin formation and likely plays a widespread role in cell adhesion and motility. VASP may also be involved in the intracellular signaling pathways that regulate integrin-extracellular matrix interactions. VASP is regulated by the cyclic nucleotide-dependent kinases PKA and PKG.
Purification Method:
Affinity purification
Gene ID:
7408
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of lysates from HEL cells, using VASP Rabbit pAb (CAB14217) at 1:800 dilution.HEL cells were treated by Forskolin (10 uM) at 37℃ for 1 hour after serum-starvation overnight.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 3s.
