The VARS2 Polyclonal Antibody (CAB15484) is a valuable tool for researchers studying the VARS2 protein, an essential enzyme involved in protein synthesis and mitochondrial function. This antibody, produced in rabbits, demonstrates high reactivity with human samples and is validated for use in Western blot applications. By specifically binding to the VARS2 protein, this antibody allows for the detection and analysis of VARS2 in various cell types, making it ideal for investigations in mitochondrial biology, metabolism, and related diseases.VARS2, also known as valyl-tRNA synthetase 2, plays a crucial role in protein synthesis by catalyzing the attachment of valine to its cognate tRNA.
Dysregulation of VARS2 has been implicated in various mitochondrial disorders and related pathologies, highlighting its importance in cellular function and health. Research into the mechanisms and functions of VARS2 can provide valuable insights into mitochondrial biology and potential therapeutic targets for mitochondrial-related diseases.By utilizing the VARS2 Polyclonal Antibody (CAB15484), researchers can further explore the role of VARS2 in mitochondrial function, metabolism, and disease pathogenesis. Its high reactivity and specificity make it a valuable tool for studies aiming to uncover the intricate mechanisms underlying VARS2 biology and its implications in human health.
Product Name:
VARS2 Rabbit Polyclonal Antibody
SKU:
CAB15484
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 864-1063 of human VARS2 (NP_065175.4).
This gene encodes a mitochondrial aminoacyl-tRNA synthetase, which catalyzes the attachment of valine to tRNA(Val) for mitochondrial translation. Mutations in this gene cause combined oxidative phosphorylation deficiency-20, and are also associated with early-onset mitochondrial encephalopathies. Alternative splicing of this gene results in multiple transcript variants.
Purification Method:
Affinity purification
Gene ID:
57176
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of lysates from U-251MG cells, using VARS2 Rabbit pAb (CAB15484) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s.
