Anti-VARS Antibody (CAB4182)

Antibody Name:	Anti-VARS Antibody
Antibody SKU:	CAB4182
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-280 of human VARS (NP_006286.1).

Application:	WB IHC IF
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:100 IF 1:50 - 1:100
Reactivity:	Human, Mouse, Rat
Positive Samples:	HepG2, HL-60, K-562, SW620, Mouse brain, Mouse heart, Mouse liver, Rat liver

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-280 of human VARS (NP_006286.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MSTL YVSP HPDA FPSL RALI AARY GEAG EGPG WGGA HPRI CLQP PPTS RTPF PPPR LPAL EQGP GGLW VWGA TAVA QLLW PAGL GGPG GSRA AVLV QQWV SYAD TELI PAAC GATL PALG LRSS AQDP QAVL GALG RALS PLEE WLRL HTYL AGEA PTLA DLAA VTAL LLPF RYVL DPPA RRIW NNVT RWFV TCVR QPEF RAVL GEVV LYSG ARPL SHQP GPEA PALP KTAA QLKK EAKK REKL EKFQ QKQK IQQQ QPPP GEKK PKPE KREK RDPG VITY
Gene ID:	7407
Uniprot:	P26640
Cellular Location:
Calculated MW:	33kDa/140kDa
Observed MW:	140kDa

Synonyms:

VARS, G7A, VARS1, VARS2

Background:

Aminoacyl-tRNA synthetases catalyze the aminoacylation of tRNA by their cognate amino acid. Because of their central role in linking amino acids with nucleotide triplets contained in tRNAs, aminoacyl-tRNA synthetases are thought to be among the first proteins that appeared in evolution. The protein encoded by this gene belongs to class-I aminoacyl-tRNA synthetase family and is located in the class III region of the major histocompatibility complex.

UniProt Protein Function:	VARS: Aminoacyl-tRNA synthetases catalyze the aminoacylation of tRNA by their cognate amino acid. Because of their central role in linking amino acids with nucleotide triplets contained in tRNAs, aminoacyl-tRNA synthetases are thought to be among the first proteins that appeared in evolution. The protein encoded by this gene belongs to class-I aminoacyl-tRNA synthetase family and is located in the class III region of the major histocompatibility complex. [provided by RefSeq, Jul 2008]
UniProt Protein Details:	Protein type:EC 6.1.1.9; Translation; Ligase; Amino Acid Metabolism - valine, leucine and isoleucine biosynthesis Chromosomal Location of Human Ortholog: 6p21.3 Cellular Component: mitochondrion; cytosol Molecular Function:valine-tRNA ligase activity; ATP binding Biological Process: tRNA aminoacylation for protein translation; regulation of translational fidelity; gene expression; valyl-tRNA aminoacylation
NCBI Summary:	Aminoacyl-tRNA synthetases catalyze the aminoacylation of tRNA by their cognate amino acid. Because of their central role in linking amino acids with nucleotide triplets contained in tRNAs, aminoacyl-tRNA synthetases are thought to be among the first proteins that appeared in evolution. The protein encoded by this gene belongs to class-I aminoacyl-tRNA synthetase family and is located in the class III region of the major histocompatibility complex. [provided by RefSeq, Jul 2008]
UniProt Code:	P26640
NCBI GenInfo Identifier:	12644177
NCBI Gene ID:	7407
NCBI Accession:	P26640.4
UniProt Secondary Accession:	P26640,Q5JQ90, Q96E77, Q9UQM2, B0V1N1, B4DZ61,
UniProt Related Accession:	P26640
Molecular Weight:	1264
NCBI Full Name:	Valine--tRNA ligase
NCBI Synonym Full Names:	valyl-tRNA synthetase
NCBI Official Symbol:	VARS
NCBI Official Synonym Symbols:	G7A; VARS1; VARS2
NCBI Protein Information:	valine--tRNA ligase; valRS; protein G7a; valyl-tRNA synthetase 2; valine tRNA ligase 1, cytoplasmic
UniProt Protein Name:	Valine--tRNA ligase
UniProt Synonym Protein Names:	Protein G7a; Valyl-tRNA synthetase; ValRS
Protein Family:	Valine--tRNA ligase
UniProt Gene Name:	VARS
UniProt Entry Name:	SYVC_HUMAN

	Western blot analysis of extracts of various cell lines, using VARS antibody (CAB4182) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s.
	Immunohistochemistry of paraffin-embedded rat brain using VARS antibody (CAB4182) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded human breast cancer using VARS antibody (CAB4182) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded mouse brain using VARS antibody (CAB4182) at dilution of 1:100 (40x lens).
	Immunofluorescence analysis of L929 cells using VARS Polyclonal Antibody (CAB4182) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)