The Valine tRNA Ligase Polyclonal Antibody (CAB4182) is a valuable tool for researchers studying the valine tRNA ligase protein in various biological processes. This antibody, produced in rabbits, exhibits high specificity and sensitivity for detecting valine tRNA ligase in human samples. Valine tRNA ligase is an essential enzyme involved in protein synthesis, specifically in attaching valine to tRNA molecules. This process is crucial for proper translation of genetic information into functional proteins, making valine tRNA ligase an important target for research in molecular biology and biochemistry.
The CAB4182 antibody is validated for use in Western blot applications, allowing for accurate and reliable detection of valine tRNA ligase in different cell types and tissues. Researchers can utilize this antibody to investigate the role of valine tRNA ligase in various physiological and pathological conditions, leading to a better understanding of protein synthesis and potential therapeutic targets in diseases related to protein translation.
Product Name:
VARS Rabbit Polyclonal Antibody
SKU:
CAB4182
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-280 of human VARS (NP_006286.1).
Aminoacyl-tRNA synthetases catalyze the aminoacylation of tRNA by their cognate amino acid. Because of their central role in linking amino acids with nucleotide triplets contained in tRNAs, aminoacyl-tRNA synthetases are thought to be among the first proteins that appeared in evolution. The protein encoded by this gene belongs to class-I aminoacyl-tRNA synthetase family and is located in the class III region of the major histocompatibility complex.
Purification Method:
Affinity purification
Gene ID:
7407
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of various lysates using VARS Rabbit pAb (CAB4182) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 30s.