The Vitamin A ELISA Kit is designed for the quantitative detection of Vitamin A levels in various biological samples. Vitamin A is essential for numerous biological mechanisms, including vision, immunity, growth, and cellular differentiation. It plays a critical role in maintaining the health of visual system and epithelial cells. Under or over levels of Vitamin A has been implicated in several diseases such as night blindness, hyperkeratosis, and toxicity. Accurate measurement of Vitamin A is vital for understanding its role in these disease mechanisms and for developing appropriate therapeutic strategies.
Assay Genie's Vitamin A ELISA Kit provides exceptional sensitivity and specificity, ensuring reliable and reproducible results. Manufactured under stringent quality control standards, this kit ensures robust performance and is user-friendly, making it an ideal choice for both research and clinical applications. Trust Assay Genie's Vitamin A ELISA Kit for accurate and dependable quantification of this vital biomarker in your studies.
Product Name:
VA (Vitamin A) ELISA Kit
SKU:
AEES00010
Target:
VA (Vitamin A)
Size:
96T
Assay type:
Competitive-ELISA
Assay time:
2.5h
Sensitivity:
9.38 ng/mL
Detection range:
15.63-1000 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with VA. During the reaction, VA in the sample or standard competes with a fixed amount of VA on the solid phase supporter for sites on the Biotinylated Detection Ab specific to VA. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of VA in tested samples can be calculated by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
89-96
89-98
98-105
Average (%)
92
95
102
1:4
Range (%)
89-104
91-105
96-106
Average (%)
97
99
101
1:8
Range (%)
99-108
84-96
89-102
Average (%)
105
90
95
1:16
Range (%)
84-95
86-99
83-95
Average (%)
92
93
91
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
99-111
104
EDTA plasma (n=5)
90-104
98
Cell culture media (n=5)
91-99
95
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
41.24
111.72
435.17
48.23
106.85
497.43
Standard deviation
2.07
5.31
22.98
2.54
5.03
23.03
C V (%)
5.02
4.75
5.28
5.27
4.71
4.63
Sample type &Sample volume:
serum, plasma and other biological fluids; 50μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of VA concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes VA in samples. No significant cross-reactivity or interference between VA and analogues was observed.
