The UVSSA Polyclonal Antibody (PACO60733) is a vital tool for researchers studying UV-stimulated scaffold protein A (UVSSA), a key player in the DNA repair pathway. This antibody, produced in rabbits, exhibits high reactivity specifically against UVSSA in human samples and is suitable for Western blot applications. By targeting the UVSSA protein, this antibody allows for accurate detection and analysis in a variety of cell types, making it an excellent option for investigations in DNA repair mechanisms and related fields.
UVSSA, known for its role in resolving UV-induced DNA damage, plays a crucial role in maintaining genomic stability and preventing the development of mutations that could lead to diseases such as cancer. Understanding the functions and interactions of UVSSA is critical for advancing research on DNA repair mechanisms and developing targeted therapies for conditions related to DNA damage. The UVSSA Polyclonal Antibody provides a valuable tool for researchers exploring these vital processes.
Antibody Name:
UVSSA Antibody (PACO60733)
Antibody SKU:
PACO60733
Size:
50ug
Host Species:
Rabbit
Tested Applications:
ELISA, IHC
Recommended Dilutions:
ELISA:1:2000-1:10000, IHC:1:200-1:500
Species Reactivity:
Human
Immunogen:
Recombinant Human UV-stimulated scaffold protein A protein (143-206AA)
IHC image of PACO60733 diluted at 1:400 and staining in paraffin-embedded human breast cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Background:
Factor involved in transcription-coupled nucleotide excision repair (TC-NER) in response to UV damage. TC-NER allows RNA polymerase II-blocking lesions to be rapidly removed from the transcribed strand of active genes. Acts by promoting stabilization of ERCC6 by recruiting deubiquitinating enzyme USP7 to TC-NER complexes, preventing UV-induced degradation of ERCC6 by the proteasome. Interacts with the elongating form of RNA polymerase II (RNA pol IIo) and facilitates its ubiquitination at UV damage sites, leading to promote RNA pol IIo backtracking to allow access to the nucleotide excision repair machinery. Not involved in processing oxidative damage.
Synonyms:
UV-stimulated scaffold protein A, UVSSA, KIAA1530
UniProt Protein Function:
UniProt Protein Details:
NCBI Summary:
The protein encoded by this gene appears to be involved in ubiquitination and dephosphorylation of RNA polymerase II subunits that stall after UV irradiation. The encoded protein interacts with several members of the nucleotide excision repair complex, and is thought to be involved in the transcription-coupled nucleotide excision repair (TC-NER) pathway to help remove lesions in the DNA that block transcription. Defects in this gene can cause UV-sensitive syndrome 3. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2015]
