The USP7 Polyclonal Antibody (PAC020830) is a crucial tool for research involving USP7, a deubiquitinase enzyme that plays a key role in regulating various cellular processes, including DNA damage response, cell cycle progression, and oncogenesis. This antibody, generated in rabbits, exhibits high specificity and sensitivity towards human samples and has been validated for use in Western blot and immunohistochemistry applications.USP7, also known as Ubiquitin Specific Protease 7, is a promising target for cancer therapy due to its involvement in the stabilization of key oncoproteins, such as Mdm2 and PTEN. Inhibitors targeting USP7 have shown potential as anticancer agents by promoting the degradation of these oncoproteins and inducing tumor cell death.
Research on USP7 has also revealed its role in various cellular pathways, making it a versatile target for drug discovery in cancer and other diseases.The USP7 Polyclonal Antibody (PAC020830) enables precise detection and quantification of USP7 protein levels in different cell types and tissue samples. Its application in research can provide valuable insights into the molecular mechanisms underlying USP7 function and its implications in disease pathogenesis. By studying USP7 with this antibody, researchers can contribute to the development of targeted therapies for cancer and other USP7-related disorders.
Antibody Name:
USP7 Antibody (PACO20830)
Antibody SKU:
PACO20830
Size:
50ul
Host Species:
Rabbit
Tested Applications:
ELISA, IHC
Recommended Dilutions:
ELISA:1:2000-1:5000, IHC:1:25-1:100
Species Reactivity:
Human, Mouse, Rat
Immunogen:
Synthetic peptide of human USP7
Form:
Liquid
Storage Buffer:
-20°C, pH7.4 PBS, 0.05% NaN3, 40% Glycerol
Purification Method:
Antigen affinity purification
Clonality:
Polyclonal
Isotype:
IgG
Conjugate:
Non-conjugated
The image on the left is immunohistochemistry of paraffin-embedded Human esophagus cancer tissue using PACO20830(USP7 Antibody) at dilution 1/30, on the right is treated with synthetic peptide. (Original magnification: x200).
Background:
Hydrolase that deubiquitinates target proteins such as FOXO4, p53/TP53, MDM2, ERCC6, DNMT1, UHRF1, PTEN and DAXX. Together with DAXX, prevents MDM2 self-ubiquitination and enhances the E3 ligase activity of MDM2 towards p53/TP53, thereby promoting p53/TP53 ubiquitination and proteasomal degradation. Deubiquitinates p53/TP53 and MDM2 and strongly stabilizes p53/TP53 even in the presence of excess MDM2, and also induces p53/TP53-dependent cell growth repression and apoptosis. Deubiquitination of FOXO4 in presence of hydrogen peroxide is not dependent on p53/TP53 and inhibits FOXO4-induced transcriptional activity. In association with DAXX, is involved in the deubiquitination and translocation of PTEN from the nucleus to the cytoplasm, both processes that are counteracted by PML. Involved in cell proliferation during early embryonic development. Involved in transcription-coupled nucleotide excision repair (TC-NER) in response to UV damage: recruited to DNA damage sites following interaction with KIAA1530/UVSSA and promotes deubiquitination of ERCC6, preventing UV-induced degradation of ERCC6. Contributes to the overall stabilization and trans-activation capability of the herpesvirus 1 trans-acting transcriptional protein ICP0/VMW110 during HSV-1 infection. Involved in maintenance of DNA methylation via its interaction with UHRF1 and DNMT1: acts by mediating deubiquitination of UHRF1 and DNMT1, preventing their degradation and promoting DNA methylation by DNMT1. Exhibits a preference towards 'Lys-48'-linked Ubiquitin chains.
Synonyms:
ubiquitin specific peptidase 7 (herpes virus-associated)
UniProt Protein Function:
USP7: an enzyme that removes ubiquitin from its protein substrates. Binds to the herpes virus protein VMW110 that may therefore modulate its substrate specificity or activity to stabilize viral proteins. Regulates MDM2 and has a dynamic role in the p53-MDM2 pathway. Is reported to deubiquitinate and stabilize p53.
UniProt Protein Details:
Protein type:Ubiquitin-specific protease; Protease; EC 3.4.19.12; Ubiquitin conjugating system
