The USP5 Polyclonal Antibody (CAB4202) is a vital tool for researchers studying USP5, a deubiquitinating enzyme involved in protein regulation and cell signaling pathways. This rabbit-derived antibody has high specificity for human samples and is validated for use in Western blotting applications. By binding to the USP5 protein, this antibody enables accurate detection and analysis in a variety of cell types, making it ideal for investigations in the fields of molecular biology, biochemistry, and cancer research.USP5, also known as ubiquitin-specific protease 5, plays a crucial role in maintaining protein homeostasis by regulating the degradation of ubiquitinated proteins. Its functions in the ubiquitin-proteasome system have implications in various cellular processes, including DNA repair, cell cycle progression, and apoptosis.
Research into USP5 is essential for understanding its potential roles in disease mechanisms, such as cancer development, neurodegenerative disorders, and viral infections.By utilizing the USP5 Polyclonal Antibody (CAB4202), researchers can further explore the intricate functions of USP5 and its impact on cellular pathways. This antibody serves as a valuable tool for investigating the molecular mechanisms underlying disease pathogenesis and identifying potential therapeutic targets for the development of novel treatments.
Product Name:
USP5 Rabbit Polyclonal Antibody
SKU:
CAB4202
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-280 of human USP5 (NP_003472.2).
Ubiquitin (see MIM 191339)-dependent proteolysis is a complex pathway of protein metabolism implicated in such diverse cellular functions as maintenance of chromatin structure, receptor function, and degradation of abnormal proteins. A late step of the process involves disassembly of the polyubiquitin chains on degraded proteins into ubiquitin monomers. USP5 disassembles branched polyubiquitin chains by a sequential exo mechanism, starting at the proximal end of the chain (Wilkinson et al., 1995 [PubMed 7578059]).
Purification Method:
Affinity purification
Gene ID:
8078
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of various lysates using USP5 Rabbit pAb (CAB4202) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 1s.
