The USP45 Polyclonal Antibody (PAC017401) is specially developed for researchers studying USP45, a protein involved in various cellular functions such as protein recycling and DNA repair. This antibody is produced using rabbit immunization and is highly reactive with human samples, making it a reliable tool for Western blot analyses. By binding to the USP45 protein, this antibody enables precise detection and analysis in a variety of cell types, making it ideal for investigations in cell biology and cancer research.USP45, also known as a deubiquitinating enzyme, plays a crucial role in maintaining cellular homeostasis by regulating protein turnover and DNA damage repair.
Its involvement in these processes makes USP45 a key player in cancer development and progression. Furthermore, understanding the function of USP45 can provide insights into potential therapeutic targets for cancer treatment and other diseases related to defective cellular processes. With its high specificity and sensitivity, the USP45 Polyclonal Antibody is a valuable tool for researchers looking to explore the role of USP45 in various cellular pathways and disease mechanisms. It offers a reliable means of studying the function and regulation of USP45, ultimately advancing our understanding of its implications in biomedical research.
Antibody Name:
USP45 Antibody (PACO17401)
Antibody SKU:
PACO17401
Size:
50ul
Host Species:
Rabbit
Tested Applications:
ELISA, IHC
Recommended Dilutions:
ELISA:1:2000-1:5000, IHC:1:25-1:100
Species Reactivity:
Human, Mouse
Immunogen:
Full length fusion protein
Form:
Liquid
Storage Buffer:
-20°C, pH7.4 PBS, 0.05% NaN3, 40% Glycerol
Purification Method:
Antigen affinity purification
Clonality:
Polyclonal
Isotype:
IgG
Conjugate:
Non-conjugated
The image on the left is immunohistochemistry of paraffin-embedded Human esophagus cancer tissue using PACO17401(USP45 Antibody) at dilution 1/25, on the right is treated with fusion protein. (Original magnification: x200).
The image on the left is immunohistochemistry of paraffin-embedded Human prostate cancer tissue using PACO17401(USP45 Antibody) at dilution 1/25, on the right is treated with fusion protein. (Original magnification: x200).
Background:
The ubiquitin (Ub) pathway involves three sequential enzymatic steps that facilitate the conjugation of Ub and Ub-like molecules to specific protein substrates. A wide range of enzymes facilitate the proteolytic Ub pathway, including USPs (ubiquitin specific peptidases), which are cysteine proteases that are responsible for the release of ubiquitin from a ubiquitylated substrate and precursor fusion proteins. USP45 (Ubiquitin carboxyl-terminal hydrolase 45), also known as Deubiquitinating enzyme 45, is a 819 amino acid, protein that is involved in ubiquitin-dependent protein catabolism. USP45 differs from other USPs in that it contains a UBP-type zinc finger, a domain which binds ubiquitin. Although USP45 is broadly expressed, the highest levels can be found in skeletal muscle, spleen and ovary. There are three isoforms of USP45 which are produced as a result of alternative splicing.
Synonyms:
ubiquitin specific peptidase 45
UniProt Protein Function:
USP45: Belongs to the peptidase C19 family. 3 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:
Protein type:EC 3.4.19.12; Ubiquitin-specific protease; Protease; Ubiquitin conjugating system
