The USP14 Polyclonal Antibody (CAB16643) is a valuable tool for researchers studying USP14, a deubiquitinating enzyme involved in protein degradation and cellular processes. This antibody, produced in rabbits, is highly specific to human samples and has been validated for use in Western blot applications.USP14, also known as ubiquitin-specific protease 14, plays a critical role in regulating the ubiquitin-proteasome system, which is responsible for targeting proteins for degradation. Dysregulation of USP14 has been implicated in various diseases, including neurodegenerative disorders and cancer.
By targeting USP14 with this antibody, researchers can better understand its function and potential therapeutic applications.This antibody enables the detection and analysis of USP14 in a variety of cell types, making it an ideal choice for studies in molecular biology, biochemistry, and cancer research. By exploring the role of USP14 in protein quality control and cell signaling pathways, researchers can gain crucial insights into disease mechanisms and potential drug targets.
Product Name:
USP14 Rabbit Polyclonal Antibody
SKU:
CAB16643
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 400-500 of human USP14 (NP_005142.1).
This gene encodes a member of the ubiquitin-specific processing (UBP) family of proteases that is a deubiquitinating enzyme (DUB) with His and Cys domains. This protein is located in the cytoplasm and cleaves the ubiquitin moiety from ubiquitin-fused precursors and ubiquitinylated proteins. Mice with a mutation that results in reduced expression of the ortholog of this protein are retarded for growth, develop severe tremors by 2 to 3 weeks of age followed by hindlimb paralysis and death by 6 to 10 weeks of age. Alternate transcriptional splice variants, encoding different isoforms, have been characterized.
Purification Method:
Affinity purification
Gene ID:
9097
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using USP14 Rabbit pAb (CAB16643) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit (AbGn00021).Exposure time: 2min.
