The USP12 Polyclonal Antibody (CAB17862) is a vital tool for researchers studying USP12, a deubiquitinating enzyme involved in protein regulation and cellular processes. This antibody, produced in rabbits, exhibits high specificity and sensitivity when detecting USP12 in human samples, making it ideal for Western blot applications. By binding to the USP12 protein, this antibody enables precise detection and analysis in a range of cell types, facilitating research in fields such as biochemistry and cell biology.
USP12, a key player in the ubiquitin-proteasome system, plays a crucial role in maintaining protein stability and regulating cellular pathways. Dysregulation of USP12 has been implicated in various diseases, including cancer and neurodegenerative disorders, making it a promising therapeutic target. By unraveling the functions of USP12, researchers can gain valuable insights into disease mechanisms and potentially develop novel treatments targeting this enzyme.
Product Name:
USP12 Rabbit Polyclonal Antibody
SKU:
CAB17862
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 1-100 of human USP12 (NP_872294.2).
Sequence:
MEIL MTVS KFAS ICTM GANA SALE KEIG PEQF PVNE HYFG LVNF GNTC YCNS VLQA LYFC RPFR EKVL AYKS QPRK KESL LTCL ADLF HSIA TQKK KVGV
Tested Applications:
WBELISA
Recommended Dilution:
WB,1:500 - 1:1000
Synonyms:
UBH1; USP12L1; USP12
Positive Sample:
A-375,A-549,mouse brain
Conjugate:
Unconjugated
Cellular Localization:
cytosol, nucleoplasm, nucleus
Calculated MW:
43kDa
Observed MW:
42kDa
Enables cysteine-type endopeptidase activity and thiol-dependent deubiquitinase. Involved in protein deubiquitination. Predicted to be located in nucleoplasm. Predicted to be active in cytosol and nucleus.
Purification Method:
Affinity purification
Gene ID:
219333
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using USP12 Rabbit pAb (CAB17862) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit (AbGn00021).Exposure time: 2min.
