URI1 Antibody (PACO55866)
- SKU:
- PACO55866
- Product Type:
- Antibody
- Reactivity:
- Human
- Host Species:
- Rabbit
- Isotype:
- IgG
- Applications:
- ELISA
- WB
- IHC
- IF
- Antibody Type:
- Polyclonal Antibody
- Conjugation:
- Unconjugated
Description
URI1 Antibody (PACO55866)
The URI1 Antibody (PAC055866) is a valuable tool for researchers studying URI1, a key protein involved in various cellular processes including cell growth and differentiation. This polyclonal antibody, produced in rabbits, is specifically designed for use in Western blot applications and has been validated for its reactivity with human samples.URI1, also known as unconventional prefoldin RPB5 interactor 1, is a critical player in regulating gene expression and protein synthesis, making it an essential target for research in cell biology and molecular biology. By binding to URI1, this antibody allows for the detection and analysis of URI1 protein in various cell types, facilitating studies in cancer research, developmental biology, and gene regulation.
The significance of URI1 in cellular processes underscores its potential as a therapeutic target for diseases such as cancer, where dysregulation of gene expression and protein synthesis contributes to tumorigenesis. By studying the function and regulation of URI1, researchers can gain insights into potential therapeutic strategies that target this protein for the treatment of cancer and other related conditions.
| Antibody Name: | URI1 Antibody (PACO55866) |
| Antibody SKU: | PACO55866 |
| Size: | 50ug |
| Host Species: | Rabbit |
| Tested Applications: | ELISA, WB, IHC, IF |
| Recommended Dilutions: | ELISA:1:2000-1:10000, WB:1:500-1:5000, IHC:1:200-1:500, IF:1:50-1:200 |
| Species Reactivity: | Human |
| Immunogen: | Recombinant Human Unconventional prefoldin RPB5 interactor 1 protein (180-298AA) |
| Form: | Liquid |
| Storage Buffer: | Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 |
| Purification Method: | >95%, Protein G purified |
| Clonality: | Polyclonal |
| Isotype: | IgG |
| Conjugate: | Non-conjugated |
 | Western Blot. Positive WB detected in: U87 whole cell lysate. All lanes: URI1 antibody at 5.3µg/ml. Secondary. Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 60, 52, 57, 54 kDa. Observed band size: 52 kDa. |
 | IHC image of PACO55866 diluted at 1:300 and staining in paraffin-embedded human adrenal gland tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. |
 | IHC image of PACO55866 diluted at 1:300 and staining in paraffin-embedded human salivary gland tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. |
| Background: | Involved in gene transcription regulation. Acts as a transcriptional repressor in concert with the corepressor UXT to regulate androgen receptor (AR) transcription. May act as a tumor suppressor to repress AR-mediated gene transcription and to inhibit anchorage-independent growth in prostate cancer cells. Required for cell survival in ovarian cancer cells. Together with UXT, associates with chromatin to the NKX3-1 promoter region. Antagonizes transcriptional modulation via hepatitis B virus X protein. |
| Synonyms: | Unconventional prefoldin RPB5 interactor 1 (Protein NNX3) (Protein phosphatase 1 regulatory subunit 19) (RNA polymerase II subunit 5-mediating protein) (RPB5-mediating protein), URI1, C19orf2 NNX3 PPP1R19 RMP URI |
| UniProt Protein Function: | RMP: Involved in gene transcription regulation. Acts as a transcriptional repressor in concert with the corepressor UXT to regulate androgen receptor (AR) transcription. May act as a tumor suppressor to repress AR-mediated gene transcription and to inhibit anchorage-independent growth in prostate cancer cells. Required for cell survival in ovarian cancer cells. Together with UXT, associates with chromatin to the NKX3-1 promoter region. Antagonizes transcriptional modulation via hepatitis B virus X protein. Homodimer. Component of the URI complex that contains PFDN2, POLR2E/RPB5, RUVBL2, RUVBL1 and URI1. Interacts with PPP1CC; the interaction is phosphorylation-dependent and occurs in a growth factor-dependent manner. Interacts with PFDN2, PFDN4 and STAP1; the interactions are phosphorylation-dependent and occurs in a growth-dependent manner in the mitochondrion. Interacts (via the middle C-terminus region) with GTF2F1 and GTF2F2. Interacts with DMAP1, POLR2E/RPB5 and UXT. Ubiquitous. Expressed in ovarian cancers. Expressed strongly in skeletal muscle. Expressed weakly in brain, heart, pancreas and in prostate epithelial cells. Belongs to the RNA polymerase II subunit 5-mediating protein family. 3 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:Transcription, coactivator/corepressor Chromosomal Location of Human Ortholog: 19q12 Cellular Component: mitochondrion; dendrite; cytoplasm; DNA-directed RNA polymerase II, core complex; nucleus Molecular Function:phosphatase inhibitor activity; protein binding; chromatin binding; protein phosphatase inhibitor activity Biological Process: regulation of transcription from RNA polymerase II promoter; transcription, DNA-dependent; response to virus; regulation of cell growth; negative regulation of transcription from RNA polymerase II promoter |
| NCBI Summary: | This gene encodes member of the prefoldin family of molecular chaperones. The encoded protein functions as a scaffolding protein and plays roles in ubiquitination and transcription, in part though interactions with the RNA polymerase II subunit RPB5. This gene may play a role in multiple malignancies including ovarian cancer and hepatocellular carcinoma. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene, and a pseudogene of this gene is located on the long arm of chromosome 22. [provided by RefSeq, Nov 2011] |
| UniProt Code: | O94763 |
| NCBI GenInfo Identifier: | 239938855 |
| NCBI Gene ID: | 8725 |
| NCBI Accession: | O94763.3 |
| UniProt Secondary Accession: | O94763,Q8TC23, Q9UNU3, A8K805, H7BY42, |
| UniProt Related Accession: | O94763 |
| Molecular Weight: | 535 |
| NCBI Full Name: | Unconventional prefoldin RPB5 interactor 1 |
| NCBI Synonym Full Names: | URI1, prefoldin-like chaperone |
| NCBI Official Symbol: | URI1Â Â |
| NCBI Official Synonym Symbols: | RMP; URI; NNX3; C19orf2; PPP1R19Â Â |
| NCBI Protein Information: | unconventional prefoldin RPB5 interactor 1; RPB5-mediating protein; protein phosphatase 1 regulatory subunit 19; protein phosphatase 1, regulatory subunit 19; RNA polymerase II subunit 5-mediating protein |
| UniProt Protein Name: | Unconventional prefoldin RPB5 interactor 1 |
| UniProt Synonym Protein Names: | Protein NNX3; Protein phosphatase 1 regulatory subunit 19; RNA polymerase II subunit 5-mediating protein; RPB5-mediating protein |
| Protein Family: | Unconventional prefoldin RPB5 interactor |
| UniProt Gene Name: | URI1Â Â |
| UniProt Entry Name: | RMP_HUMAN |
| Antibodies |
| URI1 Antibody (PACO05616) |
| Secondary Antibody |
| Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014) |
| Recommended Products |
| Anti-FITC Goat Anti-Rabbit IgG (H+L) Antibody (CABS011) |
| Anti-HRP-conjugated Beta Actin Antibody (CABC028) |
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