The URI1 Antibody (PAC023476) is a valuable tool for researchers studying the URI1 protein, which is known for its role in gene regulation and cell growth. This polyclonal antibody, raised in rabbits, is highly specific and reactive with human samples, making it an ideal choice for studies in molecular biology and cancer research.URI1 is a key player in various cellular processes, including cell proliferation, differentiation, and apoptosis. It is often dysregulated in cancer cells, making it a promising target for therapeutic intervention.
The URI1 Antibody binds specifically to the URI1 protein, enabling researchers to analyze its expression and function in different cell types and experimental conditions.Further research on the URI1 protein could provide insights into its mechanisms of action and potential therapeutic strategies for cancer treatment. By utilizing the URI1 Antibody in Western blot applications, researchers can deepen their understanding of this important protein and its implications in cellular physiology and disease progression.
Synthesized peptide derived from C-terminal of human RMP.
Form:
Liquid
Storage Buffer:
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Purification Method:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Clonality:
Polyclonal
Isotype:
IgG
Conjugate:
Non-conjugated
Western blot analysis of extracts from NIH/3T3 cells, using RMP antibody.
Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using RMP antibody.
Background:
Involved in gene transcription regulation. Acts as a transcriptional repressor in concert with the corepressor UXT to regulate androgen receptor (AR) transcription. May act as a tumor suppressor to repress AR-mediated gene transcription and to inhibit anchorage-independent growth in prostate cancer cells. Required for cell survival in ovarian cancer cells. Together with UXT, associates with chromatin to the NKX3-1 promoter region. Antagonizes transcriptional modulation via hepatitis B virus X protein. Plays a central role in maintaining S6K1 signaling and BAD phosphorylation under normal growth conditions thereby protecting cells from potential deleterious effects of sustained S6K1 signaling. The URI1-PPP1CC complex acts as a central component of a negative feedback mechanism that counteracts excessive S6K1 survival signaling to BAD in response to growth factors. Mediates inhibition of PPP1CC phosphatase activity in mitochondria. Coordinates the regulation of nutrient-sensitive gene expression availability in a mTOR-dependent manner. Seems to be a scaffolding protein able to assemble a prefoldin-like complex that contains PFDs and proteins with roles in transcription and ubiquitination.
Synonyms:
NNX3; RNA polymerase II subunit 5-mediating protein; RNA polymerase II; subunit 5-mediating protein; RPB5-mediating protein
UniProt Protein Function:
RMP: Involved in gene transcription regulation. Acts as a transcriptional repressor in concert with the corepressor UXT to regulate androgen receptor (AR) transcription. May act as a tumor suppressor to repress AR-mediated gene transcription and to inhibit anchorage-independent growth in prostate cancer cells. Required for cell survival in ovarian cancer cells. Together with UXT, associates with chromatin to the NKX3-1 promoter region. Antagonizes transcriptional modulation via hepatitis B virus X protein. Homodimer. Component of the URI complex that contains PFDN2, POLR2E/RPB5, RUVBL2, RUVBL1 and URI1. Interacts with PPP1CC; the interaction is phosphorylation-dependent and occurs in a growth factor-dependent manner. Interacts with PFDN2, PFDN4 and STAP1; the interactions are phosphorylation-dependent and occurs in a growth-dependent manner in the mitochondrion. Interacts (via the middle C-terminus region) with GTF2F1 and GTF2F2. Interacts with DMAP1, POLR2E/RPB5 and UXT. Ubiquitous. Expressed in ovarian cancers. Expressed strongly in skeletal muscle. Expressed weakly in brain, heart, pancreas and in prostate epithelial cells. Belongs to the RNA polymerase II subunit 5-mediating protein family. 3 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:
Protein type:Transcription, coactivator/corepressor
Molecular Function:phosphatase inhibitor activity; protein binding; chromatin binding; protein phosphatase inhibitor activity
Biological Process: regulation of transcription from RNA polymerase II promoter; transcription, DNA-dependent; response to virus; regulation of cell growth; negative regulation of transcription from RNA polymerase II promoter
NCBI Summary:
This gene encodes member of the prefoldin family of molecular chaperones. The encoded protein functions as a scaffolding protein and plays roles in ubiquitination and transcription, in part though interactions with the RNA polymerase II subunit RPB5. This gene may play a role in multiple malignancies including ovarian cancer and hepatocellular carcinoma. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene, and a pseudogene of this gene is located on the long arm of chromosome 22. [provided by RefSeq, Nov 2011]
