Urease Recombinant Protein (RPPB2412)
- SKU:
- RPPB2412
- Product Type:
- Recombinant Protein
- Research Area:
- Enzymes
Description
| Product Name: | Urease Recombinant Protein |
| Product Code: | RPPB2412 |
| Size: | 5mg |
| Target: | Urease |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile Lyophilized Powder. |
| Formulation: | Each mg of protein contains 420�g Potassium Phosphate and 30�g EDTA Na2. |
| Solubility: | It is recommended to reconstitute the lyophilized Urease in sterile 18M?-cm H2O. |
| Stability: | Urease although stable at 4°C for 3 weeks, should be stored desiccated below -18°C. Please prevent freeze-thaw cycles. |
| Purity: | Greater than 95.0% as determined by�SDS-PAGE. |
| Biological Activity: | The activity was found to be 141U/mg powder. |
The mutant Urease from microorganism source, showing shifted substrate affinity to urea. It was designed wildtype coding gene from microorganism.�The subunit structure is very similar to well known microbial urease.Please refer to published literature such as JBC 262, 5963-67 (1987).It is composed of multi-subunits and shows a bit complex protein structure (alpha 2 Beta 4 Gamma 4) as compared to plant ureaserUrease is genetically designed unique mutant having shifted high Km to urea, which is suited material to kinetic urea assay with wide measurable range.The enzyme comprises of three different subunits to make complete fully active form, 60.3 kD a subunit, 11.7 kD b subunit and 11.1 kD g subunit respectively.
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