The UPF3B Polyclonal Antibody (CAB15184) is a powerful tool for research involving UPF3B, a key player in mRNA surveillance and nonsense-mediated mRNA decay. This antibody, generated in rabbits, is highly specific for human samples and has been validated for use in Western blot applications. It targets the UPF3B protein, allowing for precise detection and analysis in a variety of cell types, making it an essential tool for studies in RNA biology and gene expression regulation.UPF3B is crucial for maintaining the integrity of the cellular transcriptome by ensuring the degradation of erroneous mRNA transcripts. Dysregulation of UPF3B has been linked to various diseases, including neurological disorders and cancer, highlighting its importance in cellular homeostasis.
By investigating the function of UPF3B, researchers can gain valuable insights into disease mechanisms and potentially discover novel therapeutic targets for these conditions.In summary, the UPF3B Polyclonal Antibody (CAB15184) provides researchers with a reliable tool for studying the role of UPF3B in mRNA surveillance and disease pathogenesis. Its specificity and sensitivity make it an essential component of any research project aiming to unravel the complexities of gene expression regulation.
Product Name:
UPF3B Rabbit Polyclonal Antibody
SKU:
CAB15184
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Mouse
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-180 of human UPF3B (NP_075386.1).
This gene encodes a protein that is part of a post-splicing multiprotein complex involved in both mRNA nuclear export and mRNA surveillance. The encoded protein is one of two functional homologs to yeast Upf3p. mRNA surveillance detects exported mRNAs with truncated open reading frames and initiates nonsense-mediated mRNA decay (NMD). When translation ends upstream from the last exon-exon junction, this triggers NMD to degrade mRNAs containing premature stop codons. This protein binds to the mRNA and remains bound after nuclear export, acting as a nucleocytoplasmic shuttling protein. It forms with Y14 a complex that binds specifically 20 nt upstream of exon-exon junctions. This gene is located on the long arm of chromosome X. Two splice variants encoding different isoforms have been found for this gene.
Purification Method:
Affinity purification
Gene ID:
65109
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using UPF3B Rabbit pAb (CAB15184) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 10s.
