The UPF3A Polyclonal Antibody (CAB15893) is a valuable tool for researchers studying UPF3A, a key player in mRNA surveillance and nonsense-mediated mRNA decay pathways. This antibody, raised in rabbits, is highly specific for human samples and has been validated for use in Western blot applications. By binding to the UPF3A protein, this antibody allows for the detection and analysis of UPF3A in a variety of cell types, making it ideal for research in molecular biology and genetic disorders.UPF3A is essential for the recognition and degradation of aberrant mRNA, ensuring the quality control of gene expression. Dysregulation of UPF3A has been implicated in various genetic diseases, including neurodevelopmental disorders and cancer.
Studying the function of UPF3A can provide insights into the mechanisms underlying these conditions and may lead to the development of novel therapeutic strategies.With its high reactivity and specificity, the UPF3A Polyclonal Antibody (CAB15893) is a valuable tool for researchers investigating the role of UPF3A in mRNA surveillance and genetic disorders. By enabling the detection and analysis of UPF3A in various cell types, this antibody can help advance our understanding of the molecular mechanisms governing gene expression and may pave the way for the development of targeted therapies for genetic diseases.
Product Name:
UPF3A Rabbit Polyclonal Antibody
SKU:
CAB15893
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 327-476 of human UPF3A (NP_075387.1).
This gene encodes a protein that is part of a post-splicing multiprotein complex involved in both mRNA nuclear export and mRNA surveillance. The encoded protein is one of two functional homologs to yeast Upf3p. mRNA surveillance detects exported mRNAs with truncated open reading frames and initiates nonsense-mediated mRNA decay (NMD). When translation ends upstream from the last exon-exon junction, this triggers NMD to degrade mRNAs containing premature stop codons. This protein binds to the mRNA and remains bound after nuclear export, acting as a nucleocytoplasmic shuttling protein. It forms with Y14 a complex that binds specifically 20 nt upstream of exon-exon junctions. This gene is located on the long arm of chromosome 13. Several splice variants encoding different isoforms have been found for this gene.
Purification Method:
Affinity purification
Gene ID:
65110
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of lysates from mouse testis, using UPF3A Rabbit pAb (CAB15893) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 3s.
