The UPF2 Polyclonal Antibody (CAB13411) is a valuable tool for researchers studying UPF2, a key player in the RNA quality control pathway. This antibody, generated in rabbits, exhibits high reactivity with human samples and is validated for use in Western blot applications. By binding specifically to the UPF2 protein, this antibody allows for the precise detection and analysis of UPF2 in various cell types, making it ideal for investigations in molecular biology and RNA biology research.
UPF2 is a crucial factor in the nonsense-mediated mRNA decay (NMD) pathway, which serves to eliminate aberrant mRNA transcripts and maintain cellular homeostasis. Dysregulation of UPF2 function has been implicated in a variety of diseases, including neurological disorders and cancer. By elucidating the role of UPF2 in RNA quality control mechanisms, researchers can gain valuable insights into disease processes and potentially identify new therapeutic targets for intervention.
Product Name:
UPF2 Rabbit Polyclonal Antibody
SKU:
CAB13411
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1153-1272 of human UPF2 (NP_542166.1).
WB,1:500 - 1:2000 IHC-P,1:50 - 1:200 IP,0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
Synonyms:
HUPF2; RENT2; smg-3; UPF2
Positive Sample:
Jurkat,293T
Conjugate:
Unconjugated
Cellular Localization:
Cytoplasm, perinuclear region.
Calculated MW:
148kDa
Observed MW:
170kDa
This gene encodes a protein that is part of a post-splicing multiprotein complex involved in both mRNA nuclear export and mRNA surveillance. mRNA surveillance detects exported mRNAs with truncated open reading frames and initiates nonsense-mediated mRNA decay (NMD). When translation ends upstream from the last exon-exon junction, this triggers NMD to degrade mRNAs containing premature stop codons. This protein is located in the perinuclear area. It interacts with translation release factors and the proteins that are functional homologs of yeast Upf1p and Upf3p. Two splice variants have been found for this gene; both variants encode the same protein.
Purification Method:
Affinity purification
Gene ID:
26019
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of various lysates, using UPF2 Rabbit pAb (CAB13411) at 1:2000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 180s.
