The Umps Polyclonal Antibody (CAB5492) is a valuable tool for researchers studying Uridine Monophosphate Synthetase (UMPS), an enzyme involved in the de novo pyrimidine biosynthesis pathway. This antibody, generated in rabbits, exhibits high specificity and sensitivity for detecting UMPS in human samples, making it an ideal choice for Western blot applications.UMPS plays a crucial role in nucleotide metabolism, catalyzing the conversion of orotate to UMP, a key step in pyrimidine biosynthesis. Dysregulation of this pathway has been implicated in various diseases, including cancer and metabolic disorders.
By targeting UMPS with this polyclonal antibody, researchers can gain insights into the role of this enzyme in disease pathogenesis and potentially identify novel therapeutic targets.With its proven efficacy in detecting UMPS in various cell types, the Umps Polyclonal Antibody (CAB5492) is a valuable asset for studies in biochemistry, cancer research, and drug development. Its ability to specifically recognize UMPS makes it a valuable tool for unraveling the complex mechanisms underlying nucleotide metabolism and its implications for human health.
Product Name:
UMPS Rabbit Polyclonal Antibody
SKU:
CAB5492
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 181-480 of human UMPS (NP_000364.1).
This gene encodes a uridine 5'-monophosphate synthase. The encoded protein is a bifunctional enzyme that catalyzes the final two steps of the de novo pyrimidine biosynthetic pathway. The first reaction is carried out by the N-terminal enzyme orotate phosphoribosyltransferase which converts orotic acid to orotidine-5'-monophosphate. The terminal reaction is carried out by the C-terminal enzyme OMP decarboxylase which converts orotidine-5'-monophosphate to uridine monophosphate. Defects in this gene are the cause of hereditary orotic aciduria. Alternate splicing results in multiple transcript variants.
Purification Method:
Affinity purification
Gene ID:
7372
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of extracts of Jurkat cells, using UMPS antibody (CAB5492) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s.
