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ULK1/ULK2 Rabbit Polyclonal Antibody (CAB18685)

SKU:
CAB18685
Product Type:
Antibody
Reactivity:
Human
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Cell Biology
€139 - €419
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Description

system_update_altDatasheetsystem_update_altMSDS
Product Name:ULK1/ULK2 Rabbit Polyclonal Antibody
SKU:CAB18685
Size:20uL, 100uL
Isotype:IgG
Host Species:Rabbit
Reactivity:Human
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-283 of human ULK1/ULK2 (NP_003556.1).
Sequence:MEPG RGGT ETVG KFEF SRKD LIGH GAFA VVFK GRHR EKHD LEVA VKCI NKKN LAKS QTLL GKEI KILK ELKH ENIV ALYD FQEM ANSV YLVM EYCN GGDL ADYL HAMR TLSE DTIR LFLQ QIAG AMRL LHSK GIIH RDLK PQNI LLSN PAGR RANP NSIR VKIA DFGF ARYL QSNM MAAT LCGS PMYM APEV IMSQ HYDG KADL WSIG TIVY QCLT GKAP FQAS SPQD LRLF YEKN KTLV PTIP RETS APLR QLLL ALLQ RNHK DRMD FDEF FHHP FLDA SPS
Tested Applications:WB ELISA
Recommended Dilution:WB,1:500 - 1:2000
Positive Sample:RD,SH-SY5Y,HepG2
Cellular Localization:Atg1/ULK1 kinase complex, autophagosome, cytoplasm, cytosol, mitochondrial outer membrane, recycling endosome
Observed MW:113kDa

Mammalian ULK1 (unc-51 like kinase 1) and ULK2, Caenorhabditis elegans UNC-51, and Drosophila melanogaster Atg1 are serine/threonine kinases that regulate flux through the autophagy pathway in response to various types of cellular stress.ULK1 and ULK2 all have different tissue-specific expressions partially contributing to diverse and ULK-specific interaction networks in various tissues.ULK1 (or its close family member ULK2) interacts with several other proteins to form a large complex that is required for the formation of an autophagosome. The other proteins that complex with ULK1/ULK2 include ATG13, ATG101, and RB1CC1 (FIP200/ATG17).AMPK, activated during low nutrient conditions, directly phophorylates ULK1 at multiple sites including Ser317, Ser555, and Ser777. Conversely, mTOR, which is a regulator of cell growth and is an inhibitor of autophagy, phosphorylates ULK1 at Ser757 and disrupts the interaction between ULK1 and AMPK.

Purification Method:Affinity purification
Gene ID:8408/9706
Storage Buffer:Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Product ImageWestern blot analysis of various lysates using ULK1/ULK2 Rabbit pAb (CAB18685) at 1:500 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s.

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