The UGT1A1 Polyclonal Antibody (CAB1359) is a valuable tool for researchers studying UGT1A1, an enzyme involved in the metabolism of various drugs and toxins in the liver. Raised in rabbits, this antibody is highly specific to human samples and is validated for use in Western blot applications.UGT1A1 plays a crucial role in the detoxification and elimination of potentially harmful substances from the body by conjugating them with glucuronic acid. Mutations in the UGT1A1 gene can lead to conditions like Gilbert syndrome, a disorder characterized by elevated levels of unconjugated bilirubin in the blood.
By targeting the UGT1A1 protein, researchers can gain insights into the mechanisms underlying drug metabolism and toxicity, as well as the role of this enzyme in various diseases and conditions. This antibody is a valuable tool for studies in pharmacology, toxicology, and liver diseases, providing researchers with a reliable means of detecting and analyzing UGT1A1 expression in different experimental settings.
Product Name:
UGT1A1 Rabbit Polyclonal Antibody
SKU:
CAB1359
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-200 of human UGT1A1 (NP_000454.1).
This gene encodes a UDP-glucuronosyltransferase, an enzyme of the glucuronidation pathway that transforms small lipophilic molecules, such as steroids, bilirubin, hormones, and drugs, into water-soluble, excretable metabolites. This gene is part of a complex locus that encodes several UDP-glucuronosyltransferases. The locus includes thirteen unique alternate first exons followed by four common exons. Four of the alternate first exons are considered pseudogenes. Each of the remaining nine 5' exons may be spliced to the four common exons, resulting in nine proteins with different N-termini and identical C-termini. Each first exon encodes the substrate binding site, and is regulated by its own promoter. The preferred substrate of this enzyme is bilirubin, although it also has moderate activity with simple phenols, flavones, and C18 steroids. Mutations in this gene result in Crigler-Najjar syndromes types I and II and in Gilbert syndrome.
Purification Method:
Affinity purification
Gene ID:
54658
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using UGT1A1 Rabbit pAb (CAB1359) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit (AbGn00021).Exposure time: 180s.
