The UCP3 Polyclonal Antibody (CAB16995) is a valuable tool for researchers studying Uncoupling Protein 3 (UCP3), a mitochondrial protein involved in regulating energy metabolism and thermogenesis. This antibody, generated in rabbits, exhibits high specificity and sensitivity towards human samples, making it suitable for various research applications such as Western blotting and immunohistochemistry.UCP3 is particularly important in maintaining energy balance and body weight regulation, making it a potential target for studies related to obesity, diabetes, and metabolic disorders.
By targeting UCP3 with this antibody, researchers can investigate its expression levels, subcellular localization, and functional roles in different tissues and cell types.With its ability to detect and quantify UCP3 protein, the CAB16995 antibody is a valuable asset for researchers interested in understanding the molecular mechanisms underlying metabolic processes and exploring potential therapeutic strategies for metabolic diseases.
Product Name:
UCP3 Rabbit Polyclonal Antibody
SKU:
CAB16995
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 20-119 of human UCP3 (NP_073714.1).
Mitochondrial uncoupling proteins (UCP) are members of the larger family of mitochondrial anion carrier proteins (MACP). UCPs separate oxidative phosphorylation from ATP synthesis with energy dissipated as heat, also referred to as the mitochondrial proton leak. UCPs facilitate the transfer of anions from the inner to the outer mitochondrial membrane and the return transfer of protons from the outer to the inner mitochondrial membrane. They also reduce the mitochondrial membrane potential in mammalian cells. The different UCPs have tissue-specific expression; this gene is primarily expressed in skeletal muscle. This gene's protein product is postulated to protect mitochondria against lipid-induced oxidative stress. Expression levels of this gene increase when fatty acid supplies to mitochondria exceed their oxidation capacity and the protein enables the export of fatty acids from mitochondria. UCPs contain the three solcar protein domains typically found in MACPs. Two splice variants have been found for this gene.
Purification Method:
Affinity purification
Gene ID:
7352
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using UCP3 Rabbit pAb (CAB16995) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 3s.
