UCP3 Monoclonal Antibody (CAB23285)
- SKU:
- CAB23285
- Product Type:
- Antibody
- Antibody Type:
- Monoclonal Antibody
- Reactivity:
- Mouse
- Host Species:
- Rabbit
- Isotype:
- IgG
- Applications:
- WB
Description
| Product Name: | UCP3 Monoclonal Antibody |
| SKU: | CAB23285 |
| Size: | 20uL, 100uL |
| Isotype: | IgG |
| Host Species: | Rabbit |
| Reactivity: | Mouse |
| Immunogen: | A synthesized peptide derived from human UCP3. |
| Sequence: | MVGL KPSD VPPT MAVK FLGA GTAA CFAD LVTF PLDT AKVR LQIQ GENQ AVQT ARLV QYRG VLGT ILTM VRTE GPCS PYNG LVAG LQRQ MSFA SIRI GLYD SVKQ VYTP KGAD NSSL TTRI LAGC TTGA MAVT CAQP TDVV KVRF QASI HLGP SRSD RKYS GTMD AYRT IARE EGVR GLWK GTLP NIMR NAIV NCAE VVTY DILK EKLL DYHL LTDN FPCH FVSA FGAG FCAT VVAS PVDV VKTR YMNS PPGQ YFSP LDCM IKMV AQEG PTAF YKGF TPSF LRLG SWNV VMFV TYEQ LKRA LMKV QMLR ESPF |
| Tested Applications: | WB ELISA |
| Recommended Dilution: | WB,1:500 - 1:1000 |
| Synonyms: | SLC25A9; UCP3 |
| Positive Sample: | Mouse skeletal muscle |
| Conjugate: | Unconjugated |
| Cellular Localization: | Mitochondrion inner membrane, Multi-pass membrane protein. |
| Calculated MW: | 34kDa |
| Observed MW: | 34kDa |
Mitochondrial uncoupling proteins (UCP) are members of the larger family of mitochondrial anion carrier proteins (MACP). UCPs separate oxidative phosphorylation from ATP synthesis with energy dissipated as heat, also referred to as the mitochondrial proton leak. UCPs facilitate the transfer of anions from the inner to the outer mitochondrial membrane and the return transfer of protons from the outer to the inner mitochondrial membrane. They also reduce the mitochondrial membrane potential in mammalian cells. The different UCPs have tissue-specific expression; this gene is primarily expressed in skeletal muscle. This gene's protein product is postulated to protect mitochondria against lipid-induced oxidative stress. Expression levels of this gene increase when fatty acid supplies to mitochondria exceed their oxidation capacity and the protein enables the export of fatty acids from mitochondria. UCPs contain the three solcar protein domains typically found in MACPs. Two splice variants have been found for this gene.
| Purification Method: | Affinity purification |
| Gene ID: | 7352 |
| Clone Number: | ARC60308 |
| Storage Buffer: | Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,0.05% BSA,50% glycerol,pH7.3. |
 | Western blot analysis of Mouse skeletal muscle, using UCP3 Rabbit mAb (CAB23285) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 45s. |
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