The UCP3 Polyclonal Antibody (CAB1532) is a valuable tool for researchers studying the uncoupling protein 3 (UCP3) in various biological processes. This antibody, generated in rabbits, is highly specific to human samples and is ideal for use in Western blot applications. By detecting and binding to the UCP3 protein, this antibody allows for the analysis of UCP3 expression in different cell types, making it suitable for investigations in areas such as metabolism, energy regulation, and mitochondrial function.UCP3 is a mitochondrial protein that plays a key role in the regulation of energy metabolism by uncoupling oxidative phosphorylation. Its involvement in mitochondrial uncoupling makes it a target of interest in research related to obesity, diabetes, and exercise physiology.
By studying UCP3 expression and function, researchers can gain insights into its potential role in metabolic diseases and explore its therapeutic implications.Overall, the UCP3 Polyclonal Antibody (CAB1532) serves as a valuable tool for scientists looking to delve deeper into the functions and mechanisms of UCP3 in both normal physiological processes and disease settings. Its specificity and reliability make it a trusted reagent for investigations in the fields of metabolism, mitochondrial biology, and energy metabolism.
Product Name:
UCP3 Rabbit Polyclonal Antibody
SKU:
CAB1532
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-110 of human UCP3 (NP_073714.1).
Mitochondrial uncoupling proteins (UCP) are members of the larger family of mitochondrial anion carrier proteins (MACP). UCPs separate oxidative phosphorylation from ATP synthesis with energy dissipated as heat, also referred to as the mitochondrial proton leak. UCPs facilitate the transfer of anions from the inner to the outer mitochondrial membrane and the return transfer of protons from the outer to the inner mitochondrial membrane. They also reduce the mitochondrial membrane potential in mammalian cells. The different UCPs have tissue-specific expression; this gene is primarily expressed in skeletal muscle. This gene's protein product is postulated to protect mitochondria against lipid-induced oxidative stress. Expression levels of this gene increase when fatty acid supplies to mitochondria exceed their oxidation capacity and the protein enables the export of fatty acids from mitochondria. UCPs contain the three solcar protein domains typically found in MACPs. Two splice variants have been found for this gene.
Purification Method:
Affinity purification
Gene ID:
7352
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using UCP3 Rabbit pAb (CAB1532) at 1:500 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s.
