The UCP2 Polyclonal Antibody (CAB4178) is a valuable tool for research involving UCP2, a protein involved in regulating mitochondrial membrane potential and energy metabolism. This antibody, generated in rabbits, has high reactivity with human samples and has been validated for use in Western blot applications. By specifically binding to UCP2, this antibody enables researchers to detect and analyze the protein in a variety of cell types, making it ideal for studies in metabolism, obesity, and energy homeostasis.UCP2, or uncoupling protein 2, plays a crucial role in modulating mitochondrial function and energy balance.
Its involvement in regulating metabolism and energy expenditure makes it a promising target for research into metabolic disorders, obesity, and diabetes. Understanding the function and regulation of UCP2 is essential for developing therapies that target mitochondrial function and energy metabolism in these disease contexts.Overall, the UCP2 Polyclonal Antibody (CAB4178) is a valuable tool for researchers studying the role of UCP2 in cellular metabolism and energy homeostasis, providing insights that can lead to the development of new treatments for metabolic disorders and related conditions.
Product Name:
UCP2 Rabbit Polyclonal Antibody
SKU:
CAB4178
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 112-211 of human UCP2 (NP_003346.2).
Mitochondrial uncoupling proteins (UCP) are members of the larger family of mitochondrial anion carrier proteins (MACP). UCPs separate oxidative phosphorylation from ATP synthesis with energy dissipated as heat, also referred to as the mitochondrial proton leak. UCPs facilitate the transfer of anions from the inner to the outer mitochondrial membrane and the return transfer of protons from the outer to the inner mitochondrial membrane. They also reduce the mitochondrial membrane potential in mammalian cells. Tissue specificity occurs for the different UCPs and the exact methods of how UCPs transfer H+/OH- are not known. UCPs contain the three homologous protein domains of MACPs. This gene is expressed in many tissues, with the greatest expression in skeletal muscle. It is thought to play a role in nonshivering thermogenesis, obesity and diabetes. Chromosomal order is 5'-UCP3-UCP2-3'.
Purification Method:
Affinity purification
Gene ID:
7351
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using UCP2 Rabbit pAb (CAB4178) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 180s.
