The UCP1 Polyclonal Antibody (CAB7236) is a valuable tool for researchers studying UCP1, a mitochondrial protein involved in thermogenesis and energy expenditure in brown adipose tissue. Produced in rabbits, this antibody is highly specific to UCP1 and has been validated for use in various research applications, including Western blot and immunohistochemistry.UCP1, also known as uncoupling protein 1, plays a crucial role in heat production and energy dissipation in brown adipose tissue, making it a key target in studies related to obesity, diabetes, and metabolic disorders. The UCP1 Polyclonal Antibody provides researchers with a reliable means of detecting and analyzing UCP1 levels in different cell types and tissues, facilitating investigations into the molecular mechanisms underlying metabolic regulation and thermogenesis.
By utilizing the UCP1 Polyclonal Antibody, researchers can gain valuable insights into the function of UCP1 and its potential involvement in metabolic diseases. This antibody is a valuable resource for scientists conducting research in the fields of metabolism, obesity, and energy balance, offering a reliable tool for investigating the role of UCP1 in physiological processes and pathological conditions.
Product Name:
UCP1 Polyclonal Antibody
SKU:
CAB7236
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 100-200 of human UCP1 (NP_068605.1).
Mitochondrial uncoupling proteins (UCP) are members of the family of mitochondrial anion carrier proteins (MACP). UCPs separate oxidative phosphorylation from ATP synthesis with energy dissipated as heat, also referred to as the mitochondrial proton leak. UCPs facilitate the transfer of anions from the inner to the outer mitochondrial membrane and the return transfer of protons from the outer to the inner mitochondrial membrane. They also reduce the mitochondrial membrane potential in mammalian cells. Tissue specificity occurs for the different UCPs and the exact methods of how UCPs transfer H+/OH- are not known. UCPs contain the three homologous protein domains of MACPs. This gene is expressed only in brown adipose tissue, a specialized tissue which functions to produce heat.
Purification Method:
Affinity purification
Gene ID:
7350
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of lysates from Mouse fat, using UCP1 Rabbit pAb (CAB7236) at 1:2000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 180s.
