The UCHL3 Polyclonal Antibody (CAB8156) is a crucial tool for researchers studying UCHL3, a protein involved in various cellular processes, including protein degradation and cell cycle regulation. This antibody, produced in rabbits, exhibits high reactivity with human samples and has been validated for use in Western blot applications.UCHL3, also known as ubiquitin carboxyl-terminal hydrolase isozyme L3, plays a significant role in protein turnover by removing ubiquitin molecules from targeted proteins, thereby controlling their degradation and recycling. Dysregulation of UCHL3 has been implicated in the development of various diseases, including neurodegenerative disorders and cancer.
By targeting the UCHL3 protein, researchers can investigate its role in disease pathogenesis and potential therapeutic targeting. The UCHL3 Polyclonal Antibody enables the detection and analysis of UCHL3 in different cell types, making it an essential tool for studies in molecular biology, biochemistry, and drug discovery. Understanding the function of UCHL3 is crucial for developing new strategies to combat diseases associated with protein degradation dysregulation.
Product Name:
UCHL3 Rabbit Polyclonal Antibody
SKU:
CAB8156
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-220 of human UCHL3 (NP_005993.1).
The protein encoded by this gene is a member of the deubiquitinating enzyme family. Members of this family are proteases that catalyze the removal of ubiquitin from polypeptides and are divided into five classes, depending on the mechanism of catalysis. This protein may hydrolyze the ubiquitinyl-N-epsilon amide bond of ubiquitinated proteins to regenerate ubiquitin for another catalytic cycle. Alternative splicing results in multiple transcript variants that encode different protein isoforms.
Purification Method:
Affinity purification
Gene ID:
7347
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using UCHL3 antibody (CAB8156) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s.
