The Ubiquityl-Histone H2A (K119) Rabbit Polyclonal Antibody (CAB17952) is a valuable tool for researchers studying post-translational modifications of histones. This antibody, raised in rabbits, specifically targets histone H2A ubiquitylated at lysine 119, playing a key role in gene expression regulation and DNA repair processes.Histone ubiquitylation is a crucial epigenetic modification that influences chromatin structure and gene transcription. The detection and analysis of ubiquitylated histone H2A (K119) using this antibody allows researchers to investigate the dynamic changes in chromatin states associated with various cellular processes, including DNA damage response and gene regulation.
Validated for use in Western blot applications, this antibody provides reliable and sensitive detection of ubiquitylated histone H2A (K119) in different cell types and tissues. It is an essential tool for researchers in the fields of epigenetics, chromatin biology, and cancer research, offering insights into the functional significance of histone modifications in gene expression and cellular processes.
A synthetic ubiquitinated peptide around K119 of human HIST1H2AB (NP_734466.1).
Sequence:
LPKK T
Tested Applications:
WBELISA
Recommended Dilution:
WB,1:500 - 1:2000
Synonyms:
HIST1H2AB; Ubiquityl-Histone H2A-K119
Positive Sample:
C6
Cellular Localization:
extracellular exosome, nucleus
Calculated MW:
14kDa
Observed MW:
23kDa
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures.H2A clustered histone (H2AC) consists of several H2A family members, which function as replication-dependent histone proteins.
Purification Method:
Affinity purification
Gene ID:
3012/8329
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of lysates from C6 cells, using Ubiquityl-Histone H2A-K119 Rabbit pAb (CAB17952) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit (AbGn00021).Exposure time: 180s.
