The UBE2N Polyclonal Antibody (CAB10543) is a vital tool for researchers studying UBE2N, a key enzyme involved in the ubiquitination pathway essential for protein degradation and cellular regulation. This antibody, produced in rabbits, is highly specific to human samples and has been rigorously tested for use in Western blot applications.UBE2N, also known as Ubc13, is a critical component in the cellular process of adding ubiquitin molecules to protein targets, marking them for degradation or signaling purposes. Dysregulation of the ubiquitin pathway, including UBE2N, has been implicated in various diseases, including cancer, neurodegenerative disorders, and autoimmune conditions.
By targeting UBE2N with this antibody, researchers can gain insight into the mechanisms underlying these diseases and potentially identify new therapeutic targets. Overall, the UBE2N Polyclonal Antibody is a valuable tool for researchers investigating the role of UBE2N in cellular processes and disease pathogenesis, offering precise and reliable detection for a variety of experimental applications.
Product Name:
Ube2N/Ubc13 Rabbit Polyclonal Antibody
SKU:
CAB10543
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-152 of human Ube2N/Ubc13 (NP_003339.1).
The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. Studies in mouse suggest that this protein plays a role in DNA postreplication repair.
Purification Method:
Affinity purification
Gene ID:
7334
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using Ube2N/Ubc13 antibody (CAB10543) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 15s.
