The UBE2L3 Polyclonal Antibody (CAB4175) is a valuable tool for researchers investigating the function of UBE2L3, a key enzyme involved in the process of ubiquitination. Ubiquitination plays a crucial role in protein degradation, signal transduction, and DNA repair, making UBE2L3 a vital player in cellular processes.This polyclonal antibody, generated in rabbits, exhibits high reactivity with human samples and has been validated for use in Western blot applications. By binding specifically to UBE2L3 protein, this antibody allows for the detection and analysis of UBE2L3 in various cell types, making it ideal for studies in molecular biology, biochemistry, and cell signaling research.
The importance of UBE2L3 in regulating protein turnover and cellular signaling pathways makes it a promising target for therapeutic development in cancer, neurodegenerative diseases, and other disorders characterized by dysregulated ubiquitination. By understanding the mechanisms of UBE2L3 activity, researchers can gain valuable insights into disease pathogenesis and potentially identify new strategies for intervention.
Product Name:
UBE2L3 Rabbit Polyclonal Antibody
SKU:
CAB4175
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 73-212 of human UBE2L3 (NP_001243284.1).
The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes (E1s), ubiquitin-conjugating enzymes (E2s) and ubiquitin-protein ligases (E3s). This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. This enzyme is demonstrated to participate in the ubiquitination of p53, c-Fos, and the NF-kB precursor p105 in vitro. Several alternatively spliced transcript variants have been found for this gene.
Purification Method:
Affinity purification
Gene ID:
7332
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of various lysates using UBE2L3 Rabbit pAb (CAB4175) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit (AbGn00021).Exposure time: 60s.
