The UBE2I Rabbit Monoclonal Antibody (CAB4396) is a powerful tool for researchers studying UBE2I, also known as SUMO-1 conjugating enzyme, a protein involved in post-translational modification and regulation of various cellular processes. This monoclonal antibody, produced in rabbits, exhibits high specificity and sensitivity for UBE2I in human samples, making it an ideal choice for Western blot applications.UBE2I plays a crucial role in the SUMOylation pathway, which regulates protein function, localization, and interactions within cells. Dysregulation of SUMOylation has been implicated in various diseases, including cancer, neurodegenerative disorders, and viral infections.
By targeting UBE2I with this antibody, researchers can investigate its role in these pathologies and potentially identify new therapeutic targets.By utilizing the UBE2I Rabbit Monoclonal Antibody (CAB4396), researchers can explore the intricate mechanisms of protein SUMOylation and its impact on cellular functions. This antibody offers reliable and reproducible results, enabling detailed analysis of UBE2I expression and activity in different cell types and experimental conditions. Its utility extends to a wide range of research areas, including cancer biology, neurobiology, and infectious diseases.
Product Name:
UBE2I Rabbit Monoclonal Antibody
SKU:
CAB4396
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 1-100 of human UBE2I (P63279).
The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. Four alternatively spliced transcript variants encoding the same protein have been found for this gene.
Purification Method:
Affinity purification
Gene ID:
7329
Clone Number:
ARC0996
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,0.05% BSA,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using UBE2I Rabbit mAb (CAB4396) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 1s.
