The UBE2I Polyclonal Antibody (CAB2193) is a valuable tool for researchers studying UBE2I, a critical enzyme involved in the regulation of protein function through the process of sumoylation. This antibody, generated in rabbits, exhibits high reactivity with human samples and has been validated for Western blot applications. By binding specifically to the UBE2I protein, this antibody allows for the detection and analysis of UBE2I in various cell types, making it an ideal choice for studies in molecular biology and cellular signaling pathways.
UBE2I, also known as UBC9, is essential for the attachment of small ubiquitin-like modifier (SUMO) proteins to target substrates, thereby regulating diverse cellular processes such as DNA repair, transcriptional regulation, and protein localization. Dysregulation of UBE2I has been linked to various diseases, including cancer, neurodegenerative disorders, and viral infections. Investigating the function of UBE2I is crucial for understanding its role in disease pathogenesis and potentially identifying new therapeutic targets for intervention.
Product Name:
UBE2I Rabbit Polyclonal Antibody
SKU:
CAB2193
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-158 of human UBE2I (NP_003336.1).
The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. Four alternatively spliced transcript variants encoding the same protein have been found for this gene.
Purification Method:
Affinity purification
Gene ID:
7329
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of various lysates using (CAB2193) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 30s.
