The UBE2H Polyclonal Antibody (CAB7344) is an essential tool for research involving UBE2H, a key enzyme involved in the process of ubiquitination. This antibody, generated in rabbits, exhibits high reactivity with human samples, making it suitable for a broad range of applications including Western blotting and immunohistochemistry.UBE2H plays a crucial role in mediating the transfer of ubiquitin molecules to target proteins, marking them for degradation or altering their function. Dysregulation of ubiquitination processes has been linked to various diseases, including cancer, neurodegenerative disorders, and inflammatory conditions.
By targeting UBE2H, researchers can gain insights into the mechanisms underlying these diseases and potentially identify novel therapeutic targets.The UBE2H Polyclonal Antibody is an invaluable tool for studying ubiquitination pathways and their impact on cellular processes. Its specificity and sensitivity make it a reliable choice for researchers investigating the role of UBE2H in health and disease, paving the way for new discoveries in the field of molecular biology and drug development.
Product Name:
UBE2H Rabbit Polyclonal Antibody
SKU:
CAB7344
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-183 of human UBE2H (NP_003335.1).
The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. The encoded protein sequence is 100% identical to the mouse homolog and 98% identical to the frog and zebrafish homologs. Three alternatively spliced transcript variants have been found for this gene and they encode distinct isoforms.
Purification Method:
Affinity purification
Gene ID:
7328
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using UBE2H antibody (CAB7344) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s.
