The UBE2D2 Polyclonal Antibody (CAB10818) is a valuable tool for researchers studying the ubiquitin-conjugating enzyme E2D2, an essential component in the ubiquitination pathway. This antibody, produced in rabbits, is highly specific for human samples and has been validated for use in multiple applications, including Western blotting.UBE2D2 plays a crucial role in post-translational modification of proteins by tagging them with ubiquitin, marking them for degradation by the proteasome or regulating their activity. Dysregulation of the ubiquitination pathway has been implicated in various diseases, including cancer, neurodegenerative disorders, and cardiovascular diseases.
By targeting UBE2D2, researchers can uncover novel therapeutic targets and potential treatment strategies for these conditions.The UBE2D2 Polyclonal Antibody allows for the detection and analysis of UBE2D2 expression in different cell types, providing insights into its function and role in disease pathogenesis. Its high specificity and sensitivity make it an excellent tool for investigating the molecular mechanisms underlying ubiquitin-mediated processes, advancing our understanding of cellular homeostasis and disease development.
Product Name:
UBE2D2 Rabbit Polyclonal Antibody
SKU:
CAB10818
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-147 of human UBE2D2 (NP_003330.1).
Regulated degradation of misfolded, damaged or short-lived proteins in eukaryotes occurs via the ubiquitin (Ub)-proteasome system (UPS). An integral part of the UPS system is the ubiquitination of target proteins and covalent linkage of Ub-containing proteins to form polymeric chains, marking them as targets for 26S proteasome-mediated degradation. Ubiquitination of proteins is mediated by a cascade of enzymes which includes E1 (ubiquitin activating), E2 (ubiquitin conjugating), and E3 (ubiquitin ligases) enzymes. This gene encodes a member of the E2 enzyme family. Substrates of this enzyme include the tumor suppressor protein p53 and peroxisomal biogenesis factor 5 (PEX5). Alternative splicing results in multiple transcript variants of this gene.
Purification Method:
Affinity purification
Gene ID:
7322
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using UBE2D2 Rabbit pAb (CAB10818) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s.
