The UBE2C Polyclonal Antibody (CAB5499) is a valuable tool for researchers studying UBE2C, a key enzyme involved in the regulation of cell cycles and DNA repair processes. This antibody, produced in rabbits, exhibits high reactivity with human samples and has been validated for use in Western blotting applications. By binding specifically to the UBE2C protein, this antibody allows for accurate detection and analysis in various cell types, making it an essential component in studies related to cell biology, cancer research, and DNA damage response mechanisms.UBE2C, also known as ubiquitin-conjugating enzyme E2C, plays a crucial role in maintaining genomic stability and promoting cell proliferation.
Dysregulation of UBE2C has been linked to various types of cancer, making it a promising target for therapeutic interventions. By studying the function of UBE2C, researchers can gain insights into the molecular mechanisms underlying cancer progression and potentially identify new strategies for cancer treatment. In addition, understanding the role of UBE2C in DNA repair pathways is essential for developing novel therapies for diseases associated with DNA damage, such as neurodegenerative disorders and aging-related conditions.
Product Name:
UBE2C Rabbit Polyclonal Antibody
SKU:
CAB5499
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-179 of human UBE2C (NP_008950.1).
WB,1:100 - 1:500 IHC-P,1:50 - 1:200 IF/ICC,1:50 - 1:200 IP,0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
Synonyms:
UBCH10; dJ447F3.2; UBE2C
Positive Sample:
PC-3,NIH/3T3
Conjugate:
Unconjugated
Cellular Localization:
cytosol, nucleoplasm, nucleus, plasma membrane
Calculated MW:
20kDa
Observed MW:
21kDa
The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, ubiquitin-conjugating enzymes, and ubiquitin-protein ligases. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. The encoded protein is required for the destruction of mitotic cyclins and for cell cycle progression, and may be involved in cancer progression. Multiple transcript variants encoding different isoforms have been found for this gene. Pseudogenes of this gene have been defined on chromosomes 4, 14, 15, 18, and 19.
Purification Method:
Affinity purification
Gene ID:
11065
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates, using UBE2C Rabbit pAb (CAB5499) at 1:500 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit (AbGn00021).Exposure time: 90s.
