The UBE2A Polyclonal Antibody (CAB7744) is a valuable tool for researchers studying UBE2A, a key enzyme involved in the ubiquitination process. Ubiquitination is a post-translational modification that plays a crucial role in protein degradation, DNA repair, and cell cycle regulation. This antibody, generated in rabbits, exhibits high specificity and sensitivity towards human UBE2A protein, making it an ideal choice for Western blot applications.UBE2A, also known as Ubiquitin-conjugating enzyme E2A, is essential for mediating the transfer of ubiquitin molecules to target proteins, marking them for degradation by the proteasome.
Dysregulation of UBE2A has been implicated in various diseases, including cancer, neurodegenerative disorders, and immune system dysfunction. By studying UBE2A expression and activity, researchers can gain insights into the molecular mechanisms underlying these conditions and potentially identify novel therapeutic targets.Overall, the UBE2A Polyclonal Antibody (CAB7744) offers researchers a reliable tool for investigating UBE2A function in cellular processes and disease pathways. Its versatility and specificity make it an invaluable asset for studies in biochemistry, molecular biology, and biomedical research.
Product Name:
UBE2A Rabbit Polyclonal Antibody
SKU:
CAB7744
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-152 of human UBE2A (NP_003327.2).
The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, ubiquitin-conjugating enzymes, and ubiquitin-protein ligases. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. This enzyme is required for post-replicative DNA damage repair, and may play a role in transcriptional regulation. Mutations in this gene are associated with cognitive disability. Alternative splicing results in multiple transcript variants.
Purification Method:
Affinity purification
Gene ID:
7319
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of various lysates using UBE2A Rabbit pAb (CAB7744) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit (AbGn00021).Exposure time: 90s.
