The UBE2A Polyclonal Antibody (CAB12528) is a valuable tool for researchers studying UBE2A, an enzyme involved in the ubiquitination pathway, which plays a crucial role in protein degradation and regulation of various cellular processes. This antibody, produced in rabbits, is highly specific to human samples and has been validated for use in Western blot applications. By binding to the UBE2A protein, this antibody enables precise detection and analysis in a variety of cell types, making it an essential tool for studies in cell biology and molecular biology.UBE2A has been implicated in various diseases, including cancer and neurodegenerative disorders, highlighting its importance in cellular homeostasis and disease progression.
By studying the function and regulation of UBE2A, researchers can gain valuable insights into the molecular mechanisms underlying these conditions and potentially identify new therapeutic targets for intervention. The UBE2A Polyclonal Antibody is thus a valuable asset for advancing research in these fields and expanding our understanding of ubiquitin-mediated processes in health and disease.
Product Name:
UBE2A Rabbit Polyclonal Antibody
SKU:
CAB12528
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-152 of human UBE2A (NP_003327.2).
The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, ubiquitin-conjugating enzymes, and ubiquitin-protein ligases. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. This enzyme is required for post-replicative DNA damage repair, and may play a role in transcriptional regulation. Mutations in this gene are associated with cognitive disability. Alternative splicing results in multiple transcript variants.
Purification Method:
Affinity purification
Gene ID:
7319
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of extracts of mouse kidney, using UBE2A antibody (CAB12528) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s.
