The UBA6 Polyclonal Antibody (CAB7511) is a valuable tool for researchers studying UBA6, a protein that plays a crucial role in the ubiquitination pathway. This antibody, produced in rabbits, is highly specific for UBA6 and has been validated for use in Western blot applications. By binding to UBA6, the antibody enables accurate detection and analysis of this protein in various cell types.UBA6 is involved in the regulation of protein degradation and is essential for maintaining cellular homeostasis.
Its dysregulation has been linked to various diseases, including cancer and neurodegenerative disorders. Research on UBA6 is critical for understanding the molecular mechanisms underlying these conditions and identifying potential therapeutic targets.Overall, the UBA6 Polyclonal Antibody is a reliable tool for scientists studying the role of UBA6 in cellular processes and disease pathogenesis. Its high specificity and sensitivity make it an ideal choice for immunology and cancer research projects.
Product Name:
UBA6 Rabbit Polyclonal Antibody
SKU:
CAB7511
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-260 of human UBA6 (NP_060697.4).
WB,1:500 - 1:2000 IF/ICC,1:50 - 1:200 IP,0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
Synonyms:
E1-L2; MOP-4; UBE1L2; UBA6
Positive Sample:
U-251MG,SKOV3,293T,Mouse brain
Conjugate:
Unconjugated
Cellular Localization:
cytoplasm, cytosol, nucleus
Calculated MW:
118kDa
Observed MW:
59kDa, 118kDa
Modification of proteins with ubiquitin (UBB; MIM 191339) or ubiquitin-like proteins controls many signaling networks and requires a ubiquitin-activating enzyme (E1), a ubiquitin conjugating enzyme (E2), and a ubiquitin protein ligase (E3). UBE1L2 is an E1 enzyme that initiates the activation and conjugation of ubiquitin-like proteins (Jin et al., 2007 [PubMed 17597759]).
Purification Method:
Affinity purification
Gene ID:
55236
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of various lysates using UBA6 Rabbit pAb (CAB7511) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 30s.
