The U2AF65 Rabbit Monoclonal Antibody (CAB4552) is a powerful tool for researchers studying the U2AF65 protein, an essential splicing factor involved in pre-mRNA processing. This antibody, developed specifically for use in human samples, is highly specific and sensitive, making it suitable for applications such as Western blot and immunohistochemistry.U2AF65 is a key player in the spliceosome complex, regulating alternative splicing and influencing gene expression. Dysregulation of U2AF65 has been implicated in various diseases, including cancer and neurological disorders.
By targeting U2AF65 with this monoclonal antibody, researchers can gain valuable insights into its function and potential role in disease progression.This antibody allows for precise detection and visualization of U2AF65 protein in different cell types and tissues, providing researchers with a valuable tool for studying RNA splicing mechanisms and their implications in human health and disease. Its high specificity and sensitivity make it a reliable choice for investigations in molecular biology, oncology, and neuroscience.
Product Name:
U2AF2/U2AF65 Rabbit Monoclonal Antibody
SKU:
CAB4552
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 376-475 of human U2AF2/U2AF2/U2AF65 (P26368).
WB,1:500 - 1:1000 IHC-P,1:50 - 1:200 IP,0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
Synonyms:
U2AF65; U2AF2/U2AF65
Positive Sample:
HeLa,HT-29,Mouse lung,Mouse brain,Rat brain
Conjugate:
Unconjugated
Cellular Localization:
Nucleus.
Calculated MW:
54kDa
Observed MW:
65kDa
U2 auxiliary factor (U2AF), comprised of a large and a small subunit, is a non-snRNP protein required for the binding of U2 snRNP to the pre-mRNA branch site. This gene encodes the U2AF large subunit which contains a sequence-specific RNA-binding region with 3 RNA recognition motifs and an Arg/Ser-rich domain necessary for splicing. The large subunit binds to the polypyrimidine tract of introns early during spliceosome assembly. Multiple transcript variants have been detected for this gene, but the full-length natures of only two have been determined to date.
Purification Method:
Affinity purification
Gene ID:
11338
Clone Number:
ARC1029
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,0.05% BSA,50% glycerol,pH7.3.
Western blot analysis of various lysates using U2AF2/U2AF2/U2AF65 Rabbit mAb (CAB4552) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 1s.
