The U2AF2 Polyclonal Antibody (CAB1936) is a valuable tool for researchers studying U2AF2, a key protein involved in pre-mRNA splicing. This antibody, produced in rabbits, exhibits high reactivity with human samples and has been validated for use in Western blot applications. By binding to the U2AF2 protein, it enables the accurate detection and analysis of this important molecule in a variety of cell types.U2AF2, also known as U2AF65, plays a crucial role in the recognition of 3' splice sites during splicing, contributing to the accurate processing of RNA transcripts.
Dysregulation of U2AF2 has been implicated in various diseases, including cancer and neurodegenerative disorders, highlighting the significance of studying its function. By using the U2AF2 Polyclonal Antibody, researchers can further explore the role of U2AF2 in splicing mechanisms and its potential as a therapeutic target in disease treatment.
Product Name:
U2AF2/U2AF65 Rabbit Polyclonal Antibody
SKU:
CAB1936
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 192-471 of human U2AF2/U2AF65 (NP_001012496.1).
WB,1:500 - 1:2000 IF/ICC,1:50 - 1:100 IP,0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
Synonyms:
U2AF65; U2AF2/U2AF65
Positive Sample:
Jurkat,SW620,Mouse spleen
Conjugate:
Unconjugated
Cellular Localization:
Nucleus.
Calculated MW:
54kDa
Observed MW:
54kDa
U2 auxiliary factor (U2AF), comprised of a large and a small subunit, is a non-snRNP protein required for the binding of U2 snRNP to the pre-mRNA branch site. This gene encodes the U2AF large subunit which contains a sequence-specific RNA-binding region with 3 RNA recognition motifs and an Arg/Ser-rich domain necessary for splicing. The large subunit binds to the polypyrimidine tract of introns early during spliceosome assembly. Multiple transcript variants have been detected for this gene, but the full-length natures of only two have been determined to date.
Purification Method:
Affinity purification
Gene ID:
11338
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of various lysates using U2AF2/U2AF65 Rabbit pAb (CAB1936) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.
