The U2AF1 Polyclonal Antibody (CAB13166) is specifically designed for researchers studying U2AF1, a key protein involved in pre-mRNA splicing. This antibody, produced in rabbits, exhibits high reactivity with human samples and has been validated for use in Western blot applications. By binding to U2AF1, this antibody enables accurate detection and analysis of the protein in various cell types, making it an essential tool for studies related to RNA processing and gene expression regulation.U2AF1 is a crucial factor in splice site recognition and selection during pre-mRNA splicing, playing a vital role in determining the specificity and efficiency of this essential cellular process.
Dysregulation of U2AF1 has been implicated in various diseases, including cancer and genetic disorders, making it a promising target for therapeutic interventions. By studying U2AF1 with the help of this antibody, researchers can gain valuable insights into the mechanisms underlying these conditions and potentially identify novel treatment strategies.
Product Name:
U2AF1 Rabbit Polyclonal Antibody
SKU:
CAB13166
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 1-100 of human U2AF1 (NP_006749.1).
This gene belongs to the splicing factor SR family of genes. U2 auxiliary factor, comprising a large and a small subunit, is a non-snRNP protein required for the binding of U2 snRNP to the pre-mRNA branch site. This gene encodes the small subunit which plays a critical role in both constitutive and enhancer-dependent RNA splicing by directly mediating interactions between the large subunit and proteins bound to the enhancers. Alternatively spliced transcript variants encoding different isoforms have been identified.
Purification Method:
Affinity purification
Gene ID:
7307
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using U2AF1 Rabbit pAb (CAB13166) at 1:3000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 3s.
