The TYW5 Antibody (PACO59684) is a highly specific and sensitive tool for researchers studying the TYW5 protein, which is involved in the modification of tRNA and critical for protein translation. This polyclonal antibody, produced using advanced technology, is suitable for various applications, including immunohistochemistry and western blotting.TYW5 is essential for maintaining proper protein synthesis and cellular function, making it a key target for investigation in the fields of molecular biology and genetics. By using the TYW5 Antibody, researchers can accurately detect and analyze TYW5 expression levels in different cell types, providing valuable insights into its role in cellular processes and potential implications for various diseases.
Investigating the functions and mechanisms of TYW5 is crucial for advancing our understanding of protein synthesis and its impact on human health. The TYW5 Antibody offers researchers a reliable tool for exploring the biological functions of TYW5 and its potential as a therapeutic target in the future.
Antibody Name:
TYW5 Antibody (PACO59684)
Antibody SKU:
PACO59684
Size:
50ug
Host Species:
Rabbit
Tested Applications:
ELISA, IF
Recommended Dilutions:
ELISA:1:2000-1:10000, IF:1:50-1:200
Species Reactivity:
Human
Immunogen:
Recombinant Human tRNA wybutosine-synthesizing protein 5 protein (1-152AA)
Immunofluorescence staining of Hela cells with PACO59684 at 1:166, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Background:
tRNA hydroxylase that acts as a component of the wybutosine biosynthesis pathway. Wybutosine is a hyper modified guanosine with a tricyclic base found at the 3'-position adjacent to the anticodon of eukaryotic phenylalanine tRNA. Catalyzes the hydroxylation of 7-(a-amino-a-carboxypropyl)wyosine (yW-72) into undermodified hydroxywybutosine (OHyW*). OHyW* being further transformed into hydroxywybutosine (OHyW) by LCMT2/TYW4. OHyW is a derivative of wybutosine found in higher eukaryotes.
TYW5: tRNA hydroxylase that acts as a component of the wybutosine biosynthesis pathway. Wybutosine is a hyper modified guanosine with a tricyclic base found at the 3'-position adjacent to the anticodon of eukaryotic phenylalanine tRNA. Catalyzes the hydroxylation of 7-(a-amino-a-carboxypropyl)wyosine (yW-72) into undermodified hydroxywybutosine (OHyW*). OHyW* being further transformed into hydroxywybutosine (OHyW) by LCMT2/TYW4. OHyW is a derivative of wybutosine found in higher eukaryotes. Belongs to the TYW5 family. 2 isoforms of the human protein are produced by alternative splicing.Protein type: EC 1.14.11.42Chromosomal Location of Human Ortholog: 2q33.1Molecular Function: protein homodimerization activity; iron ion binding; oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, 2-oxoglutarate as one donor, and incorporation of one atom each of oxygen into both donors; tRNA bindingBiological Process: wybutosine biosynthetic process
