The Tyrosinase Polyclonal Antibody (CAB1254) is a vital tool for researchers studying tyrosinase, an enzyme involved in the production of melanin. Produced in rabbits, this antibody shows high reactivity with human samples and is validated for use in Western blot applications. By binding specifically to the tyrosinase protein, this antibody enables researchers to detect and analyze tyrosinase expression in various cell types, making it essential for studies in dermatology, cancer research, and more.Tyrosinase plays a crucial role in melanogenesis, the process by which melanin is produced in the skin, hair, and eyes.
Understanding the regulation of tyrosinase activity is essential for investigating conditions such as hyperpigmentation disorders, melanoma, and other skin-related diseases. By utilizing the Tyrosinase Polyclonal Antibody, researchers can uncover insights into the mechanisms underlying melanin production and explore new avenues for therapeutic interventions in pigmentation disorders and skin cancer.
Product Name:
TYR Rabbit Polyclonal Antibody
SKU:
CAB1254
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 20-340 of human Tyrosinase (NP_000363.1).
Melanosome membrane, Single-pass type I membrane protein.
Calculated MW:
60kDa
Observed MW:
80kDa
The enzyme encoded by this gene catalyzes the first 2 steps, and at least 1 subsequent step, in the conversion of tyrosine to melanin. The enzyme has both tyrosine hydroxylase and dopa oxidase catalytic activities, and requires copper for function. Mutations in this gene result in oculocutaneous albinism, and nonpathologic polymorphisms result in skin pigmentation variation. The human genome contains a pseudogene similar to the 3' half of this gene.
Purification Method:
Affinity purification
Gene ID:
7299
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3.
Western blot analysis of lysates from Rat brain, using Tyrosinase Rabbit pAb (CAB1254) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s.
