The TYR Polyclonal Antibody (CAB13608) is a valuable tool for researchers studying the TYR gene, which encodes the enzyme tyrosinase involved in melanin biosynthesis. This antibody, raised in rabbits, exhibits high reactivity with human samples and has been validated for use in Western blot applications. It specifically binds to the TYR protein, allowing for accurate detection and analysis in a variety of cell types.Tyrosinase is a critical enzyme in the melanin production process, making the TYR gene a key target for research in pigmentation disorders, skin cancer, and other melanin-related conditions.
Understanding the regulation and function of TYR is essential for developing targeted therapies and interventions for these diseases.By utilizing the TYR Polyclonal Antibody, researchers can gain valuable insights into the role of tyrosinase in melanin biosynthesis and related pathways, furthering our understanding of pigmentation disorders and offering potential therapeutic strategies for these conditions.
Product Name:
TYR Rabbit Polyclonal Antibody
SKU:
CAB13608
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 20-340 of human Tyrosinase (NP_000363.1).
Melanosome membrane, Single-pass type I membrane protein.
Calculated MW:
60kDa
Observed MW:
80kDa
The enzyme encoded by this gene catalyzes the first 2 steps, and at least 1 subsequent step, in the conversion of tyrosine to melanin. The enzyme has both tyrosine hydroxylase and dopa oxidase catalytic activities, and requires copper for function. Mutations in this gene result in oculocutaneous albinism, and nonpathologic polymorphisms result in skin pigmentation variation. The human genome contains a pseudogene similar to the 3' half of this gene.
Purification Method:
Affinity purification
Gene ID:
7299
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of lysates from rat skin, using Tyrosinase Rabbit pAb (CAB13608) at 1:3000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 10s.
