The TYMS Polyclonal Antibody (CAB10441) is an essential tool for research focused on thymidylate synthase (TYMS), an enzyme involved in DNA synthesis and cell proliferation. This antibody, produced in rabbits, demonstrates high reactivity with human samples and is validated for use in Western blot applications. By binding specifically to TYMS, researchers can detect and analyze the enzyme in various cell types, making it ideal for studies in cancer biology, drug development, and personalized medicine.TYMS is a key target in cancer treatment, as its overexpression has been linked to resistance to chemotherapy and poor patient outcomes.
By studying the expression and activity of TYMS, researchers can gain valuable insights into the mechanisms of drug resistance and potential therapeutic strategies to enhance treatment efficacy. The TYMS Polyclonal Antibody is a valuable tool for investigating TYMS function and regulation in cancer cells, paving the way for personalized treatment approaches and improved patient outcomes.
Product Name:
TYMS Rabbit Polyclonal Antibody
SKU:
CAB10441
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-313 of human TYMS (NP_001062.1).
Thymidylate synthase catalyzes the methylation of deoxyuridylate to deoxythymidylate using, 10-methylenetetrahydrofolate (methylene-THF) as a cofactor. This function maintains the dTMP (thymidine-5-prime monophosphate) pool critical for DNA replication and repair. The enzyme has been of interest as a target for cancer chemotherapeutic agents. It is considered to be the primary site of action for 5-fluorouracil, 5-fluoro-2-prime-deoxyuridine, and some folate analogs. Expression of this gene and that of a naturally occurring antisense transcript, mitochondrial enolase superfamily member 1 (GeneID:55556), vary inversely when cell-growth progresses from late-log to plateau phase. Polymorphisms in this gene may be associated with etiology of neoplasia, including breast cancer, and response to chemotherapy.
Purification Method:
Affinity purification
Gene ID:
7298
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using TYMS Rabbit pAb (CAB10441) at 1:1000 dilution.Secondary antibody:HRP Goat Anti-Rabbit IgG (H+L)(CABS014) at 1:10000 dilution.Lysates / proteins: 25 μg per lane.Blocking buffer: 3 % nonfat dry milk in TBST.Detection:ECL Basic Kit (AbGn00020).Exposuretime: 60s.
